TFome clone pUT1886

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B37 UP
Temperature (ºC)	64.7
Sequence Copy to clipboard	caccATGGCCGCCTTCTTCGCT

3' Primer

Name	B37 DN
Temperature (ºC)
Sequence Copy to clipboard	TGC CGC GCC GAC GCC GAG GAA G

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MAAFFAAAPPPAAGALASFHHEEQQRQDAAEELYHVDDDVGEGGCGAGQQGKLCARGHWRPAEDAKLKELVAQYGPQNWNLIAERLDGRSGKSCRLRWFNQLDPRINRRAFSGEEEERLLAAHRAYGNKWALIARLFPGRTDNAVKNHWHVLMARKQREHTGALRRRKPSSSPDPAPPTSHLAPVVVLHHHHHQHHAGSPPRTVAAAHAQYTAADTRAHSGGESDETASTCTTDLSLGSAAAPCFYQSGYDAAPRAAAFAPSARSAFSAPPASARHGESRSDDKASPPFFDFLGVGAA

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGCCGCCTTCTTCGCTGCTGCTCCGCCGCCGGCGGCGGGTGCTCTCGCGTCGTTCCACCACGAGGAGCAGCAGCGTCAGGACGCCGCCGAGGAGCTGTACCACGTGGACGACGACGTCGGCGAGGGCGGCTGCGGCGCAGGACAGCAGGGGAAGCTGTGCGCCAGGGGCCACTGGCGCCCCGCCGAGGACGCCAAGCTCAAGGAGCTCGTCGCGCAGTACGGGCCCCAGAACTGGAACCTCATCGCCGAGAGGCTGGACGGCAGATCAGGGAAGAGCTGCCGCCTGCGGTGGTTCAACCAGCTGGACCCGCGGATCAACCGCCGGGCCTTCTCCGGCGAGGAGGAGGAGCGGCTGCTGGCGGCGCACCGCGCGTACGGCAACAAGTGGGCGCTCATCGCGCGCCTCTTCCCCGGCCGCACCGACAACGCCGTCAAGAACCACTGGCACGTCCTCATGGCGCGCAAGCAGCGGGAGCACACCGGCGCGCTCCGCCGGCGCAAACCCTCGTCCTCGCCGGACCCGGCGCCGCCGACGTCGCACTTGGCGCCCGTCGTCGTCCTCCACCACCACCACCACCAGCACCACGCCGGCTCGCCTCCGCGCACCGTCGCCGCCGCTCACGCGCAGTACACGGCAGCGGACACCCGCGCGCACAGCGGCGGCGAGTCCGACGAGACCGCGTCCACCTGCACCACCGACCTGTCCCTCGGCTCCGCCGCCGCCCCCTGCTTCTACCAGAGTGGCTACGACGCGGCTCCGCGCGCCGCGGCGTTCGCGCCCAGCGCGCGCTCCGCCTTCTCCGCGCCGCCGGCGTCGGCGCGCCATGGCGAGTCACGCTCCGACGACAAGGCGTCGCCGCCCTTCTTCGACTTCCTCGGCGTCGGCGCGGCA