TFome clone pUT3067

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	UT-2650up
Temperature (ºC)	58.0
Sequence Copy to clipboard	caccATGTCGACTAAGAAAGTTCTTTGC

3' Primer

Name	UT-2650dn
Temperature (ºC)
Sequence Copy to clipboard	AAGATCCATCCTACCAAGGAAG

PCR Condition

Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&

Amino Acid Sequence

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MSTKKVLCKFFMHGACLKGEYCEFSHDWSDQANNVCTFYQKGACSYGSRCRYDHVKVSRNPTVPPPRPTSSTSRRASTSLQLSSYNHPPHMVHQTDSSNQRHQISVDVLAHSASKPAWRNDFQHDIVSDDGIDWSSNQNLINQTSLKPVDLPICSFAAAGNCPYGEGCPQMHGDLCATCGKLCLHPYRPDEREEHIKLCEKNHKRLVALKRSQEIECSVCLDRVLSKPTAAERKFGLLSECDHPFCIECIRNWRSNSPASGMDVNSALRACPICRKLSYYVIPSVLWYFSKEEKEEITESYKSKLKSIDCKYFDFGTGSCPFGTSCFYKHAYRDGRLEEVILRHLDADDGSTVIAKSIRLSDFLGRMDL

TFome clones do not contain stop codon at 3'end.

CDS

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ATGTCGACTAAGAAAGTTCTTTGCAAGTTCTTCATGCATGGAGCTTGCTTGAAAGGAGAGTACTGTGAATTCTCCCACGACTGGAGTGATCAAGCAAATAATGTTTGCACATTCTACCAAAAAGGTGCATGCTCGTATGGTAGCCGTTGCAGATATGACCATGTCAAAGTTTCTCGTAACCCCACAGTGCCACCACCACGCCCAACGTCAAGTACTTCAAGACGTGCATCTACTTCTCTTCAACTTTCAAGTTACAATCATCCACCTCATATGGTGCACCAAACAGATTCAAGCAACCAAAGACATCAGATATCTGTGGATGTGTTGGCACATTCTGCCAGTAAGCCTGCATGGAGAAATGATTTTCAGCATGATATTGTTTCAGATGATGGAATTGACTGGTCTTCTAATCAAAATTTGATAAACCAGACATCATTGAAACCTGTGGATCTCCCTATTTGTTCTTTTGCTGCTGCTGGTAATTGTCCTTATGGGGAAGGGTGCCCTCAAATGCATGGAGATCTGTGTGCAACCTGTGGAAAACTGTGCTTGCATCCTTATCGTCCTGATGAGAGGGAGGAGCATATCAAGTTATGTGAGAAAAATCACAAACGCCTTGTAGCTCTGAAACGCAGCCAAGAAATAGAATGCAGTGTATGTTTGGATCGTGTGCTTTCAAAGCCTACTGCTGCTGAAAGGAAGTTTGGACTCTTATCCGAATGTGATCATCCTTTCTGTATTGAATGCATTAGAAATTGGCGTAGCAATTCTCCTGCATCTGGTATGGATGTGAACTCAGCATTGAGGGCTTGTCCAATATGTCGGAAACTTTCATACTATGTCATTCCAAGTGTTCTTTGGTACTTCTCAAAGGAAGAGAAAGAGGAGATCACTGAAAGCTACAAATCCAAGCTCAAGTCTATTGATTGCAAGTACTTCGACTTTGGGACTGGCTCATGCCCCTTCGGAACAAGTTGTTTCTATAAGCATGCCTACAGAGATGGCCGCTTGGAAGAGGTAATACTGCGGCATCTTGATGCTGATGATGGAAGTACAGTAATTGCAAAAAGTATCAGGTTATCAGACTTCCTTGGTAGGATGGATCTT