TFome clone pUT3079

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	UT2520up
Temperature (ºC)	60.8
Sequence Copy to clipboard	caccATGCATCCAGGAGGTGGACCA

3' Primer

Name	UT2520dn
Temperature (ºC)
Sequence Copy to clipboard	CTTGGGGTACCTGAGGAGATCGG

PCR Condition

Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&

Amino Acid Sequence

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MHPGGGPLSVPPGFRFHPTDEELLYYYLRKKVSYEPIDLDVIREIDLNQLEPWDLKERCRIGTGHQNEWYFFSHKDKKYPTGTRTNRATTAGFWKATGRDKAIFLGNASRRIGLRKTLVFYIGRAPHGKKTDWIMHEYRLDQENVEIQEDGWVVCRVFKKKSYHQRGLNPAEMAAALDDDELQPFPVPIPGSSLQPTEHKTNPTHLMQYDYPSFDPSMQLPQLMSDDQEQEQPVRPTLLPGHPGVAMASMSSLNIECSQNLTKLTSNGSDEMLHVGHGGGAGGGVVVDRFAGTTTDWSILDKLLASHQNLDRLFHGKVVTTASASPMAVPVYHQQLMELGGSSSSLQRLPLQYLGGETADLLRYPK

TFome clones do not contain stop codon at 3'end.

CDS

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ATGCATCCAGGAGGTGGACCACTGTCGGTGCCACCAGGCTTCCGCTTCCACCCGACCGACGAGGAGCTCCTCTACTACTACCTGAGGAAGAAGGTTTCCTATGAGCCCATAGATCTTGATGTCATAAGGGAGATTGATCTCAACCAGCTTGAGCCATGGGATCTCAAAGAAAGGTGCAGGATAGGCACCGGGCATCAGAACGAGTGGTACTTCTTCAGCCACAAGGACAAGAAGTACCCGACGGGAACAAGGACGAACCGCGCCACGACGGCGGGGTTCTGGAAGGCGACTGGGCGAGACAAGGCCATCTTCCTCGGCAACGCCTCCAGGAGGATCGGCTTGAGGAAGACCCTGGTGTTCTACATCGGCAGGGCGCCACACGGCAAGAAGACTGACTGGATCATGCACGAGTACCGCCTGGACCAAGAGAACGTTGAGATTCAGGAAGATGGTTGGGTGGTGTGTAGGGTTTTCAAGAAGAAGAGCTATCACCAGAGAGGCCTCAATCCGGCTGAAATGGCCGCCGCACTGGACGACGACGAGCTCCAGCCTTTCCCTGTTCCTATCCCCGGCAGCTCCCTGCAGCCAACAGAGCACAAGACCAACCCTACTCACCTCATGCAATATGACTATCCTTCCTTCGACCCTTCCATGCAGCTCCCACAGCTGATGAGCGATGACCAGGAACAGGAACAGCCCGTGCGGCCAACCCTCCTCCCCGGCCATCCCGGCGTTGCTATGGCCTCCATGAGCTCACTCAACATTGAGTGCTCGCAGAACCTGACGAAGCTCACATCTAACGGCAGCGACGAGATGCTCCACGTCGGCCACGGCGGTGGGGCTGGAGGTGGTGTAGTCGTCGACCGCTTCGCTGGCACGACGACAGATTGGTCGATCCTAGACAAGCTCCTCGCCTCGCACCAGAACCTCGACCGGCTCTTCCACGGCAAGGTCGTCACCACGGCATCTGCCTCCCCAATGGCGGTACCGGTATACCATCAGCAGCTCATGGAACTCGGTGGCTCGTCGTCGTCCTTGCAGAGGCTTCCACTGCAGTACCTCGGCGGCGAGACGGCCGATCTCCTCAGGTACCCCAAG