TFome clone pUT3148

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B279_F
Temperature (ºC)	68.0
Sequence Copy to clipboard	caccATGGCGGCGCGTGACCAC

3' Primer

Name	B279_R
Temperature (ºC)
Sequence Copy to clipboard	CAGCCCCATTATCTGCGTGTACCAC

PCR Condition

30 sec 98°C, 35x(10sec 98°C, 30sec 58°

Amino Acid Sequence

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MAARDHRELSGDEDSVVAAGDLRRGPWTVEEDMLLVNYVAAHGEGRWNALARCAGTRCCMLVATLRDDDQTNNTMNCAYAGLRRTGKSCRLRWLNYLRPDLRRGNITAQEQLLILELHSRWGNRWSKIAQHLPGRTDNEIKNYWRTRVQKHARQLNCDVNSQRFKDLMRYIWMPRLLERIGSDDSGDGAFVIDAATPTPTPTLLTAATWCVDDVELSCTTAVSSSSSVSTDSMHQQQQQQQLVAVSPPPQPAAFGESNNDASHYGVHNSNAAMSMWGDTLCQPPHSATPHLAGAGTCSWSDESLLVPDAGGGVYADMGLPDLCDTDTMWAAGASADDDLWYTQIMGL

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGCGGCGCGTGACCACCGAGAGCTGAGCGGCGACGAGGACTCCGTGGTGGCGGCCGGAGACCTCCGCCGCGGGCCATGGACGGTGGAGGAGGACATGCTCCTCGTCAACTACGTCGCCGCGCACGGCGAGGGCCGCTGGAACGCGCTGGCACGATGCGCAGGTACGCGGTGCTGCATGCTGGTTGCCACGCTACGAGACGACGACCAGACTAATAACACAATGAACTGCGCATATGCAGGGCTCCGGCGGACGGGGAAGAGCTGCCGCCTGCGGTGGCTCAACTACCTGCGGCCGGACCTGCGGCGGGGCAACATCACGGCGCAGGAGCAGCTGCTCATCCTGGAGCTGCACTCCCGCTGGGGCAACCGCTGGTCCAAGATCGCGCAGCACCTCCCGGGGCGCACCGACAACGAGATCAAGAACTACTGGCGCACGCGGGTGCAGAAGCACGCCAGGCAGCTCAACTGCGACGTCAACAGCCAGCGCTTCAAGGACCTCATGCGCTACATCTGGATGCCGCGCCTCCTCGAGCGCATCGGCTCCGACGACTCCGGGGACGGCGCCTTCGTTATCGACGCCGCCACGCCGACGCCAACGCCGACGCTCCTCACGGCCGCCACGTGGTGCGTGGACGACGTCGAGCTGTCCTGCACCACCGCCGTGTCGTCGTCGTCGTCCGTGTCCACGGACAGCATGCACCAGCAGCAGCAGCAGCAGCAGCTCGTCGCCGTGTCCCCGCCGCCACAGCCCGCCGCCTTTGGCGAGAGCAACAACGACGCCTCCCACTACGGTGTCCACAACAGCAACGCTGCTATGTCCATGTGGGGGGACACGCTGTGCCAACCACCGCACTCGGCGACGCCGCACCTGGCGGGCGCCGGGACCTGCAGCTGGTCAGACGAGTCTCTGCTGGTACCTGATGCCGGCGGCGGCGTGTACGCCGACATGGGGCTTCCGGACCTCTGCGACACCGACACCATGTGGGCCGCCGGCGCCAGCGCCGACGACGACCTGTGGTACACGCAGATAATGGGGCTG