| PCR Condition:
|
40 sec 98°C, 30x(15sec 98°C, 30sec 45° |
| Nucleotide Sequence:
|
ATGGCCGTGGACGAGGCGAGCAGCAGTGGCGGTGGGGAGGAGGGGTGCGGGTCGTGGACCCGCGAGCAGGAGAAGGCGTTCGAGAACGCGGTGGCGACGATGGGCGGGGAGGAGGACGGGGACGCGAGGTGGGAGAAGCTAGCGGAGGCCGTCGAGGGGAAGACGCCGGAGGAGGTCAGGCGGCACTACGAGCTGCTGGTGGAGGACGTCGACGGCATCGAGTCGGGCCGCGTCCCGCTTCCGGCGTACGCGGCTGACGGCGCTGCCGAGGAAGGGGGCGGTGGCGGGAAGAAGGGAAGTGGCGGAGGAGGGACCCATGGGGACAAGGGGTCGGCGAAGTCCGCCGAGCAGGAGCGTAGGAAGGGCATCGCCTGGACAGAGGACGAGCACAGATTGTTCCTCCTTGGACTTGAAAAGTATGGCAAAGGTGACTGGCGGAGCATCTCGCGGAACTTTGTGATCTCAAGGACGCCGACTCAAGTAGCTAGTCATGCACAGAAGTACTTTATCCGTCTGAACTCAATGAACAGAGAAAGGCGGCGATCAAGTATACATGACATCACCAGTGTGAACAATGGAGATCCATCAACTGCACAGGGTCCAATCACAGGCCAAACAAATGGTCAGGCTGCAAATCCTGGAAAACCATCTAAGCAATCCCCGCAGCCAGCGAATACACCTCCAGGCGTCGATGCTTATGGCACAACAATTGGACAGCCAGTTGGTGGCCCCCTTGTCTCAGCTGTTGGAACTCCCGTTACACTACCTGTTTCCGCTCCACCTCATCTGGCCTATGGCATGCGTGCCCCGGTTCCAGGAGCCGTAGTACCTGGTGCTCCAGTAAACATTGCTCCGATGCCCTATCCAATGCCACCCCCACCATCTCATGGG |
|
|
TFome clones do not contain stop codon at 3'end.
|
| translation:
|
MAVDEASSSGGGEEGCGSWTREQEKAFENAVATMGGEEDGDARWEKLAEAVEGKTPEEVRRHYELLVEDVDGIESGRVPLPAYAADGAAEEGGGGGKKGSGGGGTHGDKGSAKSAEQERRKGIAWTEDEHRLFLLGLEKYGKGDWRSISRNFVISRTPTQVASHAQKYFIRLNSMNRERRRSSIHDITSVNNGDPSTAQGPITGQTNGQAANPGKPSKQSPQPANTPPGVDAYGTTIGQPVGGPLVSAVGTPVTLPVSAPPHLAYGMRAPVPGAVVPGAPVNIAPMPYPMPPPPSHG
|
