| PCR Condition:
|
40 sec 98°C, 35x(10sec 98°C, 30sec 58° |
| Nucleotide Sequence:
|
ATGTCCAGCGCCCTGCGCGCCGCCTGGAGCGGGGCTCAGCAGCAGCTGCCGCCCCACGCGGGCGGTGGCATCGGCGGGCATAGGGCGCAGCACCACAAGGGCCCCTGGACCGAGGCGGAGGACGTAGTCCTCAGGGAGATGGTGATGAAGCACGGCGACCGGAAGTGGGCGGTGATAGCGCAGTCGCTCCCCGGCAGGGTGGGCAAGCAGTGCCGCGAGAGATGGACCAACCACCTGCGCCCCGACCTCAAGAAGAGCGTGTGGACTGAAGAAGACGACATGGCGCTGATCAAGGCGCACAAGCGGTGCGGCAACCACTGGTCGACCATCGCCACGTTCCTGCCCGGGCGTTCCGAGAACGCCGTGAAGAACCACTGGAACGCCACCAAGCGGAGCCTCAAGGCCAAGCGCCGGCTGAAGAAGAAGAGCAACGCGCAGCAGGTGCCGGCCGGGCAGTGGTCCGTCCTGGAGGAGTACATCCGCAGCCTGTACCCTGACCTCGCCGACGGCGCCGGCGCCGACCAGACCCCGCCGGCGGCCGAGGACTCCCCCCCGTCGTCCTACAACCTCGGGTCGTACTCGTACGGCGAGGTGATCAGCAGCCCAGCGTCCGCCGCCGCGCCGCCCGGCAGCTTTGACCAGATGACCGCCATGGGGCTGTACCTGGGCGCGTCGTCGTCGTCGCCCGCGCCGGCCGAGCTGCAGGGGGGCATGAGCTCCGACATCGTGGCGCCGTTCCTGCACCTGGACCTCAACGCCTACTACGGCGGCGCGCCGGCGGCGACGATGCGGCTTATGGCTCCGATGGGGGGAATGGCGGCGCCTGTGCCGCAGATGATGGAGCACGATCACTACCAGCACCAGCAGGCTTCCTACGCCACCGCCAACCAGCTGCTCACGTACCCCTTCATCGACAACAGCGTCATGTGGCAGCAGGCGCCGTACGCCGCCCATGGCAGCGCCGCCTACGGCGGTGGCGCCGACGCGGCAGGTGCCGGCCCGAGCAACGTCGTCGCCGTTCCGGCGGACGACGTCGACGTCGTCCAGATGGCCTCCAGGGAGTTCCTGACGCCGTCGGAGGACGAGGTCACCCTGGACCTGGCCAGGTTCCGT |
|
|
TFome clones do not contain stop codon at 3'end.
|
| translation:
|
MSSALRAAWSGAQQQLPPHAGGGIGGHRAQHHKGPWTEAEDVVLREMVMKHGDRKWAVIAQSLPGRVGKQCRERWTNHLRPDLKKSVWTEEDDMALIKAHKRCGNHWSTIATFLPGRSENAVKNHWNATKRSLKAKRRLKKKSNAQQVPAGQWSVLEEYIRSLYPDLADGAGADQTPPAAEDSPPSSYNLGSYSYGEVISSPASAAAPPGSFDQMTAMGLYLGASSSSPAPAELQGGMSSDIVAPFLHLDLNAYYGGAPAATMRLMAPMGGMAAPVPQMMEHDHYQHQQASYATANQLLTYPFIDNSVMWQQAPYAAHGSAAYGGGADAAGAGPSNVVAVPADDVDVVQMASREFLTPSEDEVTLDLARFR
|
