| TFome pUT3153 | |
|---|---|
| Template | BT061882.1 |
| Genbank Insert ID | JX469909 |
| Protein Name | ZmMYB155 |
| Species | Zea mays |
| TF Family | MYB |
| Gene id | GRMZM2G150680 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT3153
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B300_F |
| Temperature (ºC) | 61.7 |
| Sequence
|
caccATGGAATCCGATGAGTC |
3' Primer
| Name | B300_R |
| Temperature (ºC) | |
| Sequence
|
ACTTGAACCTGGCCAAGCC |
PCR Condition
5 l GC buffer (NEB) 1 l primer forward 20mM 1 l pr
Amino Acid Sequence
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MESDESAFLNNMLAPPMAPDKRTKRYFASSSHGHILFEQDLLATSSMQASDAQLMGDPLEARSLVASIGMTPAGFEMPEGEILSRGYHALGGTPVDIAPAPQPQIESSNKPTAFKGPWTMEEDSILRDMVMHHGEGRWSLVAQSLPGRIGKQCRERWINHVDPNIKKNDIWTEEEDIVLIQAHKSFGSHWSTIAKFLPGWPENAIKNHWNSTKRSLGSKRRLKKKKSQQAPPGQLSVLEEYIRSLESDSSSAAPPAATSRPAIGRPRRAVHTSAVPEMELHLSAPNNQDAPVRHQPRAISQGNPMLPDLNVSYDPEETHYMGYPMYDPAAAPQLQLVSQEPRQATFTWLPFGGPRMALNGEHASGPSSYDVGGSSGNAGYYSEAGPSNVGGSGGDGQADDDTTNNDDLDLASREFFMPSCDEVRLGLARFK
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAATCCGATGAGTCTGCCTTCCTCAACAACATGCTTGCACCACCCATGGCACCCGACAAGCGAACAAAGAGGTACTTTGCAAGCTCTAGCCATGGCCATATATTGTTTGAGCAAGATCTACTGGCTACTAGTTCTATGCAAGCCAGTGATGCTCAACTCATGGGAGACCCCCTCGAGGCGAGGTCCCTTGTGGCAAGCATAGGTATGACTCCAGCAGGTTTTGAAATGCCAGAGGGGGAAATTTTATCCCGTGGCTACCACGCTCTTGGTGGTACTCCAGTCGACATTGCTCCAGCTCCTCAGCCACAAATCGAAAGTAGCAACAAGCCAACGGCTTTCAAAGGACCATGGACCATGGAAGAGGATAGCATTTTGAGGGATATGGTAATGCATCATGGTGAAGGGAGGTGGTCCCTGGTAGCACAATCTTTGCCTGGCCGGATTGGCAAGCAGTGCCGTGAAAGATGGATCAATCATGTGGATCCCAACATCAAGAAAAATGACATATGGACGGAGGAGGAGGACATAGTGCTTATCCAAGCCCACAAATCCTTTGGAAGCCACTGGTCAACAATAGCGAAGTTCCTCCCAGGCTGGCCAGAGAACGCCATCAAGAACCACTGGAACTCAACCAAGCGCAGCCTAGGCTCAAAGCGCCGACTGAAGAAGAAGAAGAGCCAACAAGCGCCACCCGGGCAGCTCTCCGTCCTGGAGGAGTACATCCGGAGCCTGGAATCGGACTCCAGTTCGGCAGCACCACCGGCGGCAACGTCCAGACCAGCCATCGGTCGTCCGAGGAGGGCCGTCCACACATCGGCGGTGCCTGAGATGGAACTGCACCTCAGTGCCCCCAATAACCAAGATGCACCGGTGCGCCACCAGCCCCGGGCCATCAGTCAAGGCAATCCGATGCTTCCAGACCTGAACGTCAGCTATGATCCAGAGGAGACGCACTATATGGGGTACCCTATGTATGATCCCGCGGCGGCGCCGCAGCTACAGCTGGTTAGCCAAGAGCCCCGGCAAGCCACCTTCACTTGGCTCCCGTTTGGTGGACCTCGCATGGCGCTGAACGGAGAGCACGCATCTGGTCCGTCGTCGTACGACGTCGGTGGATCCTCCGGCAACGCCGGCTACTACAGTGAAGCAGGGCCAAGCAACGTCGGTGGCAGCGGTGGTGACGGCCAAGCTGATGACGACACCACCAACAATGACGACCTTGACCTAGCGTCGAGGGAGTTCTTCATGCCCTCCTGTGACGAGGTGCGTCTTGGCTTGGCCAGGTTCAAG
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