| TFome pUT3155 | |
|---|---|
| Template | BT062722.1 |
| Genbank Insert ID | JX469910 |
| Protein Name | ZmMYB81 |
| Species | Zea mays |
| TF Family | MYB |
| Gene id | GRMZM2G020772 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT3155
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B311_F |
| Temperature (ºC) | 69.1 |
| Sequence
|
caccATGATGCGGGAGACGTACATGG |
3' Primer
| Name | B311_R |
| Temperature (ºC) | |
| Sequence
|
AAACAGAACGCTGTGGTGGCTG |
PCR Condition
5 l GC buffer (NEB) 1 l primer forward 20mM 1 l pr
Amino Acid Sequence
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MMRETYMDVLPPVDHIAASRSGGWFPGAARLWTAAENKQFERALAGLDLCRPDWEKVARAIPGRTVREVVSHFKSLQVDVQQIESGLVPMPVYGAGAGSFTLQWDGCYGPADSRHNGYRFGSGGCGRRHHGRTPEQDRKKGVPWTEEEHRLFLLGLKKYGKGDWRNISRNYVQTRTPTQVASHAQKYFIRLNSGGKDKRRSSIHDITTVNLTDDERAPSPSRSSLITTTSQPNAPAPAAAVIGGPFSSAAAAEAKQHQHGAGNLPPNRTSVVPAVYRMDDSQDHGLQCGPLRHQLVASHHSVLF
TFome clones do not contain stop codon at 3'end.
CDS
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ATGATGCGGGAGACGTACATGGACGTGCTGCCGCCGGTGGACCACATAGCCGCCTCACGGAGCGGCGGCTGGTTCCCGGGCGCGGCGCGTCTCTGGACGGCGGCGGAGAACAAGCAGTTCGAGCGGGCGCTGGCCGGGCTCGACCTCTGCCGCCCGGACTGGGAGAAGGTGGCGCGGGCCATCCCGGGCCGGACGGTGCGCGAGGTCGTCAGCCACTTCAAGAGCCTCCAGGTCGACGTCCAGCAGATCGAGAGCGGCCTGGTGCCCATGCCGGTCTACGGCGCCGGGGCCGGCTCGTTCACCCTGCAGTGGGACGGCTGCTACGGCCCCGCGGACTCGAGGCACAACGGCTACCGCTTCGGAAGCGGAGGCTGCGGGAGGCGGCACCACGGCCGCACCCCGGAGCAGGACCGGAAGAAGGGCGTGCCGTGGACGGAGGAGGAGCACAGGCTGTTCCTCTTAGGCCTGAAGAAATACGGCAAGGGGGACTGGAGGAACATATCGCGCAACTACGTCCAGACGCGGACGCCCACGCAGGTGGCCAGCCACGCGCAGAAGTACTTCATCAGGCTCAACTCCGGCGGCAAGGACAAGAGGAGGTCCAGCATCCACGACATCACCACGGTGAACCTGACGGACGACGAGCGGGCGCCCTCGCCGTCGCGGTCCTCTCTGATCACCACCACCAGCCAGCCAAACGCGCCGGCTCCGGCCGCAGCGGTGATAGGCGGGCCCTTCTCGTCGGCGGCGGCGGCCGAGGCCAAGCAGCACCAGCACGGCGCTGGGAACCTGCCACCGAACCGGACCTCCGTGGTGCCGGCTGTTTACAGGATGGACGATTCGCAGGACCACGGCCTGCAGTGTGGCCCTCTGCGTCATCAACTGGTCGCCAGCCACCACAGCGTTCTGTTT
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