TFome pUT3167
Template BT084336.1
Genbank Insert ID KJ726811
Protein Name ZmWRKY110
Species Zea mays
TF Family WRKY
Gene id GRMZM2G173680
transcript T01
Vector pENTR/SD/D-TOPO
Note
Request Information Deposited to ABRC
Subgenome: 2

TFome clone pUT3167

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name B355_F
Temperature (ºC) 61.6
Sequence
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caccATGGAGGAGGTGGAGGTG

3' Primer

Name B355_R
Temperature (ºC)
Sequence
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GGTCTGGGCAGGCTGTGC

PCR Condition

PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f

Amino Acid Sequence
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MEEVEVANMAAVESCHRVLALLSQQQDPALLKSVASETAEACAKFRKVAALLGSASGGGGCGHARGRFSRRVRPLGLVNQKSPLGIATGSGNPLEMMPSTAAAAGSPSPPQSTSYAQMRARLSSAPESSRGLDLACSSSRCSPHPFGAPKLVQPLSVQFQIGSVAHRYPFHQQQSRQKLQAEMFRRSNSGISLKFDSPSPSGGAAGTMSSARSFMSSLSIDRSMASLDGKRPFHLVGTPVASDPADAHRAPKRRCTGRGEDGRGKCATTGRCHCSKRRKLRIKRSIRVPAISNKIADIPPDEYSWRKYGQKPIKGSPHPRGYYKCSSVRGCPARKHVERCVDDPAMLIVTYEGEHSHTQLPAQPAQT

TFome clones do not contain stop codon at 3'end.

CDS
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ATGGAGGAGGTGGAGGTGGCCAACATGGCCGCGGTGGAGAGCTGCCACCGGGTGCTGGCTCTGCTCTCGCAGCAGCAGGACCCAGCCCTGCTCAAGAGCGTAGCTTCAGAGACGGCTGAAGCCTGCGCCAAGTTCAGGAAGGTAGCGGCCCTCCTCGGCAGTGCCAGTGGCGGAGGTGGCTGTGGCCATGCTAGAGGCAGGTTCTCCAGACGAGTCCGGCCTCTGGGGCTCGTCAACCAGAAGAGCCCCTTGGGGATCGCCACCGGCAGCGGCAACCCGCTGGAGATGATGCCCAGCACAGCTGCTGCTGCTGGGTCTCCATCTCCACCTCAGTCGACTAGCTATGCGCAAATGCGCGCTCGGCTTAGCAGTGCGCCAGAGTCCTCACGAGGGCTGGATTTGGCCTGCTCCAGCAGCAGGTGTAGCCCTCATCCATTTGGAGCCCCCAAGCTGGTCCAGCCACTGTCTGTGCAGTTCCAGATTGGGAGTGTTGCGCATAGGTACCCGTTCCACCAGCAGCAGTCGAGGCAGAAGCTGCAGGCCGAGATGTTCAGGAGGAGCAACAGTGGGATCAGCCTCAAGTTCGATAGCCCTAGCCCCAGCGGTGGTGCTGCTGGCACCATGTCGTCTGCGAGATCATTCATGTCGTCGTTGAGTATAGACAGGAGCATGGCGAGCTTGGATGGGAAGCGGCCGTTCCATTTGGTAGGCACCCCGGTGGCGAGCGACCCAGCAGATGCGCACCGTGCGCCCAAACGGCGATGCACGGGTAGAGGGGAGGATGGAAGAGGCAAGTGTGCCACCACTGGCAGATGCCATTGCTCAAAGAGAAGGAAACTGCGGATTAAGAGGTCGATTAGAGTGCCAGCCATTAGCAACAAAATCGCTGATATCCCTCCTGATGAGTACTCGTGGCGCAAGTACGGGCAGAAGCCAATTAAGGGTTCCCCACACCCGAGGGGTTACTACAAATGCAGCAGCGTCAGGGGCTGCCCAGCAAGGAAGCACGTCGAGCGATGTGTAGACGACCCGGCGATGCTAATCGTGACATACGAAGGCGAGCACAGCCACACCCAGCTGCCAGCACAGCCTGCCCAGACC


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