TFome clone pUT3178

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B442_F
Temperature (ºC)	67.6
Sequence Copy to clipboard	caccATGAAGAAGTGCCCATCGGA

3' Primer

Name	B442_R
Temperature (ºC)
Sequence Copy to clipboard	CTTGGACAGGCCGCCGTT

PCR Condition

PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f

Amino Acid Sequence

Copy to clipboard

MKKCPSELELEAFLRGREDATAAAAAAVAEQKPTHDIAALAPFGAGGVFPSSDLSAFSFADSNTLNGTGNIHNHLWSHNHNVRHPAVSTTIESQSSICAAAAASPTSATNLYLKESQALGGTSGSDSDSDSLLDIEGGPCEQSTNPQDVKRMRRMVSNRESARRSRKRKQAHLADLETQVDQLRGENASLFKQLTDANQQFTTAVTDNRILKSDVEALRVKVKLAEDMVARGALSCGLGSLGLSPALNPRQACRGPDVLSVSGLDFPGADDACFTGLSPAEQLQLQSSPLQSIAASLESLDNRMASEVTSCGGPGVDVWPWDHHSNGGLSK

TFome clones do not contain stop codon at 3'end.

CDS

Copy to clipboard

ATGAAGAAGTGCCCATCGGAGCTGGAGCTGGAGGCTTTCCTCCGCGGCCGGGAGGACGCCACGGCGGCGGCGGCGGCGGCGGTGGCCGAGCAGAAGCCTACGCACGACATCGCTGCGCTGGCGCCGTTCGGCGCCGGTGGCGTGTTCCCCTCCAGCGATCTGTCCGCCTTCAGCTTCGCTGACTCGAACACCCTCAATGGAACTGGAAACATCCATAATCACCTTTGGTCCCATAACCACAACGTCAGGCATCCCGCAGTCTCCACGACGATCGAGTCGCAGTCGTCGATCTGTGCAGCTGCAGCAGCAAGTCCAACATCGGCTACCAACCTGTACCTGAAAGAGAGCCAGGCTCTGGGCGGCACGAGTGGTTCCGACTCCGACAGCGATTCGCTGCTGGACATCGAGGGTGGCCCGTGCGAGCAGAGCACGAACCCACAGGACGTGAAGAGGATGCGACGGATGGTGTCGAACCGTGAGTCTGCTCGACGGTCCAGGAAGAGGAAGCAAGCCCACCTAGCTGACCTTGAGACACAGGTCGACCAGCTGCGAGGCGAGAACGCGTCGCTCTTCAAGCAGCTGACGGACGCCAACCAGCAGTTCACGACCGCGGTCACGGACAACAGGATCCTCAAGTCGGACGTGGAGGCCCTCAGAGTCAAGGTGAAGCTGGCGGAGGACATGGTGGCGCGCGGCGCGCTGTCGTGCGGGCTGGGCAGCCTGGGGCTGTCGCCGGCGCTGAACCCGCGGCAGGCGTGCCGCGGCCCCGACGTGCTGTCCGTGTCCGGCCTGGACTTCCCCGGCGCCGACGACGCCTGCTTCACGGGGCTGTCCCCGGCGGAGCAGCTGCAGCTCCAGAGCTCCCCCCTGCAGAGCATCGCCGCCAGCCTCGAGAGCCTGGACAACCGGATGGCCAGCGAGGTCACCAGCTGCGGGGGGCCCGGCGTCGACGTCTGGCCGTGGGACCACCACTCCAACGGCGGCCTGTCCAAG