| TFome pUT3206 | |
|---|---|
| Template | EE174967 |
| Genbank Insert ID | JX469945 |
| Protein Name | ZmMYB106 |
| Species | Zea mays |
| TF Family | MYB |
| Gene id | GRMZM2G048910 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C557T p.P186L Not in functional domain. Not in template FlcDNA. |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT3206
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C154_F |
| Temperature (ºC) | 73.5 |
| Sequence
|
caccATGGGGAGGCAGCCGTGCT |
3' Primer
| Name | C154_R |
| Temperature (ºC) | |
| Sequence
|
TATTCTGTGGTCGTCTTCTTGGTCGAA |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f
Amino Acid Sequence
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MGRQPCCDKVGVKKGPWTAEEDQKLVGFLLTHGHCCWRVVPKLAGLLRCGKSCRLRWTNYLRPDLKRGLLSDDEERLVIDLHAQLGNRWSKIAAQLPGRTDNEIKNHWNTHIRKKLVRMGIDPVTHLPLQEPPAPAPAPAEQQEQSHRQQEELQLQELQNGRELIMQEGAGEDDITPMIQPHEIMLTAAAASNCGSVSSAHAGSASVVSPSCSSSAVSGVEWPEPMYLLGMDGIMDADWGSLFPDTGAGGGGGGFDLGVDPFDQYPGGGFDQEDDHRI
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGGAGGCAGCCGTGCTGCGACAAGGTTGGCGTGAAGAAGGGCCCGTGGACGGCGGAGGAGGACCAGAAGCTCGTCGGCTTCCTCCTCACCCACGGTCACTGCTGCTGGCGCGTCGTCCCCAAGCTCGCAGGGTTGCTGAGGTGCGGGAAGAGCTGCAGGCTGCGGTGGACCAACTACCTGAGGCCGGACCTCAAGAGGGGCCTGCTCTCCGACGACGAGGAGAGGCTCGTCATCGACCTGCACGCGCAGCTCGGGAACAGGTGGTCCAAGATCGCGGCGCAGCTACCGGGCAGGACGGACAACGAGATCAAGAACCACTGGAACACACACATCCGCAAGAAGCTCGTCCGGATGGGGATCGACCCCGTCACCCACCTGCCGTTGCAGGAACCGCCGGCTCCTGCTCCTGCTCCCGCCGAGCAGCAGGAGCAGAGCCACAGGCAGCAAGAAGAACTGCAACTGCAAGAGCTGCAGAACGGCCGCGAGCTTATCATGCAGGAGGGCGCGGGGGAAGACGATATCACGCCGATGATCCAGCCGCACGAGATCATGCTGACGGCGGCAGCGGCAAGCAACTGCGGCTCTGTTTCCTCTGCCCACGCCGGCTCCGCGTCCGTGGTCTCGCCGTCCTGCTCCTCCTCGGCGGTGTCCGGCGTGGAGTGGCCGGAGCCCATGTACCTGCTCGGCATGGATGGCATCATGGACGCCGACTGGGGCAGCCTCTTCCCCGACACTGGCGCTGGCGGCGGCGGCGGCGGCTTCGACTTGGGCGTCGATCCGTTCGACCAGTACCCGGGCGGTGGCTTCGACCAAGAAGACGACCACAGAATA
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