| TFome pUT3221 | |
|---|---|
| Template | BT067773.1 |
| Genbank Insert ID | JX469959 |
| Protein Name | ZmbZIP94 |
| Species | Zea mays |
| TF Family | bZIP |
| Gene id | GRMZM2G007063 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C128T p.A43V Not present in template FlcDNA. Not in functional domain. |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT3221
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C291_F |
| Temperature (ºC) | 69.3 |
| Sequence
|
caccATGGAGCGCGTCTTCTCCAT |
3' Primer
| Name | C291_R |
| Temperature (ºC) | |
| Sequence
|
GGAGGTCGAGCCGGATGAAGC |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f
Amino Acid Sequence
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MERVFSMEEIPNPYWAPPHPQPAAGGAVAAPGGVGGAGDAAGVMNRCPSEWYFQKFLEEAVLDSPGPVAGVGRSSGQAGVEAAESKPLGAAAPASVSSSVVDPVEYNAMLKQKLEKDLAAIAMWRASGAAPPDLSATAASLPSVGAPHAAPLKPVGGTESLVQNMLAGAPVGGSGPHVVQIADIPVKQTTSSSSREQSDDDDMEGDAETNGNGNPVQQRQQRRKQSNRESARRSRSRKAAHLNELEAQVAQLRVENSSLLRRLADVNQKFNEAAVDNRVLKADVETLRAKVKMAEDSVKRVTGMNALYPAVSDMSSLSMPFNGSPSDSASDSTVPVQDDLNSYFANPSEIGCNNGYMPDIASSVQQDDNFVNGYQAAGKMGRTDSLQRVASLEHLQKRMCGGPASSGSTS
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAGCGCGTCTTCTCCATGGAGGAGATCCCCAACCCCTACTGGGCCCCGCCGCACCCTCAACCGGCGGCCGGCGGCGCTGTTGCTGCACCAGGTGGAGTAGGAGGAGCGGGGGACGCGGCGGGCGTGATGAACCGGTGCCCATCTGAGTGGTACTTCCAGAAGTTCCTCGAGGAGGCCGTGCTCGACAGTCCGGGTCCCGTCGCCGGCGTGGGTAGAAGCAGCGGACAAGCTGGAGTTGAGGCGGCGGAGAGCAAGCCGCTGGGCGCCGCGGCGCCGGCGTCCGTCTCGAGCTCGGTCGTTGACCCCGTCGAGTACAACGCGATGCTCAAGCAGAAGCTGGAGAAGGACCTCGCTGCCATCGCTATGTGGAGGGCTTCTGGTGCAGCACCTCCAGATCTTTCTGCGACTGCTGCTTCCTTGCCAAGTGTCGGTGCTCCGCATGCAGCTCCTCTTAAACCCGTCGGAGGTACTGAAAGTCTAGTTCAAAACATGCTAGCTGGTGCTCCAGTAGGGGGGTCAGGTCCACATGTAGTACAAATTGCTGATATCCCTGTTAAGCAAACCACTAGCTCTTCCTCACGAGAGCAGTCAGATGATGATGACATGGAAGGCGATGCTGAGACAAATGGAAATGGAAACCCTGTTCAACAAAGACAACAGAGAAGGAAGCAATCCAACCGGGAATCAGCCAGGCGTTCGAGAAGCAGAAAGGCGGCTCACTTGAATGAACTGGAGGCACAGGTAGCACAATTAAGAGTCGAAAACTCTTCGCTGCTAAGGCGGCTTGCTGATGTTAACCAGAAGTTCAATGAAGCTGCTGTTGACAATAGGGTGCTAAAGGCAGACGTCGAAACCTTAAGAGCAAAGGTGAAGATGGCAGAGGACTCGGTGAAGCGGGTAACAGGCATGAACGCATTGTACCCTGCCGTGTCGGATATGTCTTCCCTCAGCATGCCATTCAATGGCTCCCCTTCTGACTCCGCCTCTGATAGCACCGTCCCGGTACAAGATGACCTGAACAGTTACTTCGCCAACCCAAGCGAAATCGGATGCAACAACGGCTACATGCCAGATATAGCCTCCTCGGTTCAACAGGACGACAATTTTGTCAACGGGTATCAGGCTGCTGGCAAGATGGGCAGAACAGACTCGCTGCAGCGGGTGGCAAGCCTGGAGCACCTCCAGAAAAGAATGTGCGGAGGCCCGGCTTCATCCGGCTCGACCTCC
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