| TFome pUT3225 | |
|---|---|
| Template | BT054772.1 |
| Genbank Insert ID | JX469963 |
| Protein Name | ZmE2F16 |
| Species | Zea mays |
| TF Family | E2F-DP |
| Gene id | GRMZM2G041701 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT3225
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT2748up |
| Temperature (ºC) | 60.2 |
| Sequence
|
caccATGTCGGGGGTCGGCAGC |
3' Primer
| Name | UT2748dn |
| Temperature (ºC) | |
| Sequence
|
TGGATCTGTGCTTGGAACCTCCATAT |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MSGVGSGRPPAAQKILQSLRPPLSFSTPSRPPFAAPDDYHRFPTPAAATSPAATSGGVGAGAAPPDTVEEGLVIRTPLKRKATSEENAAADSSSCIITSPMLTPVSAKTVKASKAKAKNSKTGPQTPTSNVGSPLNPPTPVGTCRYDNSLGLLTKKFINLLKQAPDGILDLNNAAEVLEVQKRRIYDITNVLEGIGLIEKTLKNRIRWKALDDSSVQLDNGISALQAEVENLSLQEQALDERISDIREKLRGLTEDENNQRWLYVTEDDIKGLPSFQNETLIAIKAPHGTTLEVPDPDEAGDYLQRRYRIVLRSTMGPIDVYLVSQFDEKFEELGGVATPAKHSNVPRHQPAEDFNTSYAGQSSTLMGVAHDAQHSQKIPQDPSALHDFGGMTRISPSDVHTDSDYWLLTEGDVSMTDMWKTEQAQWDEMDFLSEDVVTPRAHNQQLVTVDGPHMEVPSTDP
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTCGGGGGTCGGCAGCGGCAGGCCGCCGGCAGCCCAGAAGATCCTGCAGTCCCTGCGACCGCCGCTGTCCTTCTCCACGCCGTCGCGGCCACCCTTCGCCGCGCCCGACGACTACCACCGCTTTCCAACGCCGGCTGCGGCCACGTCCCCCGCCGCCACCTCAGGCGGCGTTGGCGCGGGGGCTGCTCCTCCTGATACTGTCGAGGAGGGGCTGGTCATACGGACTCCGCTAAAAAGGAAAGCCACATCTGAAGAAAATGCTGCTGCTGACTCAAGCAGTTGTATCATTACCAGTCCAATGCTCACTCCGGTCTCTGCTAAAACTGTTAAAGCTTCTAAGGCAAAGGCTAAGAACAGTAAAACTGGGCCTCAGACACCTACATCAAATGTTGGTTCACCACTCAATCCACCAACGCCTGTTGGCACATGCCGCTATGACAATTCACTAGGACTTCTGACAAAAAAGTTCATTAACTTGCTTAAGCAAGCTCCAGATGGCATTCTAGATTTGAACAATGCTGCAGAAGTACTAGAGGTTCAAAAGCGGCGCATATATGACATTACCAATGTCCTTGAAGGCATTGGACTAATAGAGAAGACACTTAAGAATAGAATCCGTTGGAAGGCCCTGGATGATTCAAGTGTTCAATTAGATAATGGTATCTCTGCTTTGCAGGCAGAAGTTGAAAATCTTAGTCTGCAGGAGCAAGCTCTAGATGAGCGTATAAGTGACATCCGTGAAAAATTAAGGGGCTTAACTGAAGATGAAAATAATCAAAGGTGGCTCTATGTGACTGAAGACGATATCAAGGGATTACCCAGCTTTCAGAATGAAACACTAATTGCAATTAAAGCACCTCATGGTACTACTCTTGAAGTACCAGATCCTGATGAGGCAGGTGATTATCTCCAAAGGAGATATAGAATTGTATTAAGAAGCACGATGGGACCAATAGATGTTTACTTAGTTAGTCAATTTGATGAAAAATTTGAGGAACTGGGTGGTGTTGCGACACCCGCCAAGCATTCAAATGTGCCCAGACATCAACCAGCTGAAGATTTCAATACATCATATGCTGGACAAAGTAGCACACTAATGGGTGTGGCACATGATGCACAGCACAGCCAGAAGATTCCCCAGGATCCTAGTGCTTTGCATGACTTTGGAGGGATGACAAGGATTAGTCCTTCAGACGTCCATACGGATTCTGATTACTGGCTCCTAACAGAGGGGGATGTTAGCATGACTGATATGTGGAAAACAGAACAAGCGCAGTGGGATGAGATGGATTTCTTGTCAGAAGATGTTGTCACCCCTCGAGCCCATAATCAGCAGCTGGTCACAGTTGATGGGCCACATATGGAGGTTCCAAGCACAGATCCA
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