TFome pUT3371
Template BT068513
Genbank Insert ID KJ726831
Protein Name ZmGLK52
Species Zea mays
TF Family G2-like
Gene id GRMZM2G124495
transcript T01
Vector pENTR/SD/D-TOPO
Note
Request Information Deposited to ABRC
Subgenome: 2

TFome clone pUT3371

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name UT2885up
Temperature (ºC) 55.8
Sequence
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caccATGAACGCCAAGAAGATTAAGCTG

3' Primer

Name UT2885dn
Temperature (ºC)
Sequence
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GCTTATCTTCGACTGGAATCCG

PCR Condition

Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&

Amino Acid Sequence
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MNAKKIKLHDYHHCYGSPMCDPQLFPRAAAATTAGLSLHPGPGLVGSLPQRHGGGGGGWVHEEHTATTPRAAQGQGGCVVGSDAAAFFAAEELMMGTARFDSPLGGTTTALQELTAFAKGPPFGRPRPTTGGERERERLYPVDPLPLRDCAAVRTYYVRPQQRDGATEAPPSLELPFQRRQQQQVHGLFGDPSTGRLLGGGEPQAHSFPAHTLKQVPASTFVPAMEAPPGMQSLMDNPLSRSCSIIGAAATHAGSGNAAAPGQGAPSKTRIRWTQDLHERFVDCVNKLGGADKATPKGILKLMNSDGLTIYHIKSHLQKYRIAKYMPVSSTSEGKEKRAAAANDVQNLDPGTGMKITEALRVQLDVQRRLHEQLEIQRNLQLRIEAQGKKLQKMFEEQMKTSRTVMGPPQGADVAFIGAGEQEEEVEVEDAFDDVQLLAAVSSASVGYRDGGFQSKIS

TFome clones do not contain stop codon at 3'end.

CDS
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ATGAACGCCAAGAAGATTAAGCTGCACGACTATCACCACTGCTACGGATCGCCGATGTGTGACCCACAGCTGTTCCCGCGCGCCGCCGCCGCCACCACCGCAGGGCTCTCCTTGCACCCGGGGCCGGGGCTCGTGGGCTCCCTGCCGCAGCGGCATGGCGGCGGCGGCGGCGGCTGGGTGCACGAGGAGCACACCGCGACGACCCCGAGGGCGGCGCAGGGGCAGGGCGGCTGCGTCGTCGGCTCCGACGCCGCCGCGTTCTTCGCCGCCGAGGAGCTCATGATGGGCACGGCGCGGTTCGACTCCCCTCTCGGCGGAACGACGACGGCACTCCAGGAGCTGACAGCGTTTGCCAAGGGACCGCCGTTTGGCCGGCCGAGGCCCACGACGGGGGGCGAGCGCGAGCGCGAGCGGCTGTACCCAGTGGACCCGCTGCCGCTCCGCGACTGCGCGGCGGTGAGGACGTACTACGTCCGGCCGCAGCAGCGCGACGGCGCCACGGAGGCGCCTCCTTCACTCGAGCTGCCATTCCAGCGGCGGCAGCAGCAACAAGTGCACGGGCTATTCGGCGACCCTTCCACCGGCAGGCTACTCGGCGGCGGCGAGCCTCAAGCTCACTCCTTTCCAGCTCACACCCTGAAGCAGGTTCCGGCGAGCACGTTTGTCCCCGCGATGGAGGCGCCGCCGGGCATGCAGAGCCTTATGGACAACCCGCTGTCCAGGAGCTGCAGTATCATCGGTGCGGCGGCGACCCACGCAGGGAGCGGTAACGCCGCCGCGCCGGGGCAAGGTGCTCCCAGCAAGACGCGGATTCGGTGGACGCAGGACCTCCACGAGCGCTTCGTCGACTGCGTCAACAAGCTCGGCGGCGCGGACAAGGCGACTCCCAAGGGCATTCTGAAGCTGATGAACTCTGATGGCCTCACAATCTACCACATCAAGAGCCACCTTCAGAAATACCGCATAGCCAAGTACATGCCCGTGTCATCGACGTCCGAAGGGAAAGAGAAACGAGCTGCTGCCGCCAATGACGTGCAGAATCTCGACCCCGGCACCGGGATGAAGATCACGGAAGCGCTACGCGTCCAGCTCGACGTGCAGAGGCGCCTCCACGAGCAGCTCGAGATCCAGAGGAATCTGCAGCTGAGGATCGAGGCGCAAGGCAAGAAGCTGCAGAAGATGTTCGAGGAGCAGATGAAGACGAGCAGGACCGTGATGGGGCCGCCGCAGGGCGCCGACGTCGCCTTCATCGGCGCCGGCGAGCAGGAGGAGGAGGTGGAGGTGGAGGACGCGTTCGACGACGTGCAGCTACTGGCGGCCGTGTCCAGCGCCAGCGTCGGCTACCGCGACGGCGGATTCCAGTCGAAGATAAGC


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