TFome clone pUT3391

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	UT3018up
Temperature (ºC)	68.5
Sequence Copy to clipboard	caccATGGAGGTTTCGGCCGAGTGCGC

3' Primer

Name	UT3018dn
Temperature (ºC)
Sequence Copy to clipboard	GATGAGCTGACGGAAGTCCGGCCCTATG

PCR Condition

Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&

Amino Acid Sequence

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MEVSAECAGGGSVKKEADLFLVDDLLDLPCDDDDEETVVGDWEGDGSKQAAVLDRGCGAGGEEGAAGNASKNESSAVTALDSCSNSISGSGLADGDFSGGLCEPYDQLAELEWLSNYLGEDNFPTEDLKKLQLITGIPPAATAMAPAPAPAAAQAQPAAGVLPQEAPVLGKARSKRSRVAPCSWASRLVVLPPPSPGSPPSAAISPSESGSGTAALAFPARKPLKPAKKKEAPSPSLPPVPNNAAAAGAGEGRRCLHCETDKTPQWRTGPLGPKTLCNACGVRYKSGRLVPEYRPAASPTFVVSKHSNSHRKVLELQRQKEAHPHHHHQYQPQQALAHVGAGGTLNLMHAPSPLLFDGPAAPLIGDDFLVHSHIGPDFRQLI

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGAGGTTTCGGCCGAGTGCGCGGGCGGCGGGAGTGTGAAGAAGGAGGCGGACCTCTTCCTTGTCGACGACCTACTCGACCTGCCGTGCGACGACGACGACGAGGAGACGGTGGTGGGCGACTGGGAAGGGGACGGGAGTAAGCAGGCTGCGGTGTTGGACCGGGGTTGTGGCGCCGGCGGGGAGGAGGGTGCCGCCGGTAACGCGTCGAAGAACGAATCGTCGGCGGTGACTGCGCTGGACAGCTGCAGCAATTCCATCTCGGGTTCCGGCCTCGCCGACGGGGACTTCTCCGGCGGGCTGTGCGAGCCGTACGACCAACTAGCGGAGCTGGAATGGCTGTCCAACTACCTGGGCGAGGACAACTTTCCCACGGAGGATCTGAAGAAGCTGCAGCTCATCACCGGCATTCCGCCGGCTGCAACCGCGATGGCGCCCGCGCCCGCGCCCGCCGCCGCACAGGCACAGCCGGCCGCTGGCGTGTTGCCACAGGAGGCGCCCGTGCTGGGCAAGGCACGCAGCAAGCGGTCGCGCGTCGCGCCATGCAGCTGGGCCTCCCGCCTGGTCGTGCTCCCGCCACCTAGTCCCGGCTCGCCGCCGTCCGCGGCCATCTCGCCGTCCGAGTCCGGCTCCGGCACCGCGGCGCTGGCGTTCCCGGCCAGGAAGCCGTTGAAGCCTGCGAAGAAGAAGGAGGCGCCGTCGCCGTCGCTGCCGCCGGTGCCAAACAACGCCGCGGCGGCGGGCGCGGGGGAGGGGCGGCGGTGCCTGCACTGCGAGACCGACAAGACGCCTCAGTGGAGGACGGGGCCGCTGGGCCCCAAGACGCTGTGCAACGCGTGCGGCGTGCGGTACAAGTCGGGCCGCCTCGTGCCGGAGTACCGGCCGGCGGCGAGCCCGACCTTCGTGGTGTCGAAGCACTCCAACTCCCACCGGAAGGTGCTGGAGCTGCAGCGCCAGAAGGAGGCGCACCCGCACCACCACCACCAGTACCAGCCGCAGCAGGCCCTCGCCCACGTCGGCGCCGGCGGGACCTTGAACTTGATGCACGCGCCGAGCCCGCTGCTGTTCGACGGGCCGGCGGCGCCGCTCATCGGCGACGACTTCTTGGTCCACAGCCACATAGGGCCGGACTTCCGTCAGCTCATC