TFome clone pUT3402

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	UT3262up
Temperature (ºC)	70.7
Sequence Copy to clipboard	caccATGGCGCGGTGCCAGGGGAC

3' Primer

Name	UT3262dn
Temperature (ºC)
Sequence Copy to clipboard	CATTTCCATTTTGAGGTTGTCTAGCAAGCAGC

PCR Condition

Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&

Amino Acid Sequence

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MARCQGTVESLCQGLLDLDDDKFGAMCSAFGYLQEWPDLGAAMCGANLAVPPAVATPSAGDGNDSSSCSVSGGLRKRRPDAYLDAKGGANGMNKRPRGKQQPSGLSEIAAAPGKGKQERPKAGTKKKAEAAAAAAAAGQKTDYIHVRARRGQATDSHSLAERVRRERISERMRYLQELVPGCSKVTGKAGMLDEIINYVQSLQKQVEFLSMKIAASNPVVSFDIVEDLFGRQLKQACGPAAGQLEPSCLQMSMSPMQQVVHPSAAAGSTGFGPLDMVLSNLYAPAAASCPVSAAPSVSVAAGPSTIEPCLNVNGTAAAWDIGSQNLFSGFDAQFQSVESGCLLDNLKMEM

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGCGCGGTGCCAGGGGACCGTTGAGAGCCTCTGCCAGGGGCTGCTCGACCTCGACGACGACAAGTTCGGTGCAATGTGCAGCGCCTTCGGCTACCTCCAGGAGTGGCCGGACCTGGGTGCCGCCATGTGCGGCGCCAATCTCGCCGTGCCGCCTGCCGTGGCGACGCCGAGCGCCGGCGACGGCAACGATTCCTCCTCTTGCAGCGTCAGCGGCGGGTTGAGGAAGAGGAGGCCGGACGCGTACCTCGATGCCAAGGGTGGTGCGAACGGCATGAACAAGAGACCCAGAGGGAAGCAGCAGCCGTCTGGCCTCTCTGAGATCGCGGCGGCGCCCGGGAAAGGGAAGCAGGAGAGGCCAAAGGCTGGCACCAAGAAGAAGGCCGAGGCTGCGGCGGCGGCGGCGGCTGCCGGCCAGAAGACCGACTACATCCACGTCCGGGCCCGCCGCGGCCAGGCCACGGACAGCCACAGCCTCGCCGAGCGGGTGAGGAGGGAGAGGATCAGCGAGCGGATGCGGTACCTGCAGGAGCTGGTGCCCGGGTGCAGCAAGGTCACGGGCAAGGCCGGCATGCTCGACGAGATCATCAACTACGTCCAGTCCCTGCAGAAGCAAGTCGAGTTCTTGTCCATGAAGATCGCCGCTTCCAACCCGGTGGTGAGCTTCGACATCGTCGAGGACCTCTTCGGCCGCCAGCTGAAGCAGGCGTGCGGCCCGGCGGCCGGCCAGCTGGAGCCGTCCTGCCTTCAGATGAGCATGAGCCCCATGCAGCAGGTAGTGCACCCGTCTGCAGCGGCGGGCTCCACTGGCTTTGGGCCGCTGGACATGGTCCTCAGCAACCTGTACGCGCCCGCCGCCGCAAGCTGCCCTGTGTCGGCTGCCCCGTCGGTCTCGGTGGCCGCCGGACCGTCGACCATCGAGCCGTGCCTCAACGTGAATGGAACTGCTGCTGCCTGGGATATTGGCTCCCAGAATTTGTTCAGCGGATTTGATGCACAATTTCAGTCGGTAGAAAGTGGCTGCTTGCTAGACAACCTCAAAATGGAAATG