| TFome pUT3446 | |
|---|---|
| Template | BT055234.2 |
| Genbank Insert ID | KJ726901 |
| Protein Name | ZmMYB128 |
| Species | Zea mays |
| TF Family | MYB |
| Gene id | GRMZM2G017520 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT3446
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B298_F |
| Temperature (ºC) | 72.4 |
| Sequence
|
caccATGGGGAGGCACTCCTGCTGTT |
3' Primer
| Name | B298_R |
| Temperature (ºC) | |
| Sequence
|
GATATTCTCAAAAGACAAGGACATCCTTT |
PCR Condition
5 l GC buffer (NEB) 1 l primer forward 20mM 1 l pr
Amino Acid Sequence
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MGRHSCCYKQKLRKGLWSPEEDEKLMNHITKHGHGCWSTVPKLAGLQRCGKSCRLRWINYLRPDLKRGAFSEEEEDLIVELHAVLGNRWSQIATRLPGRTDNEIKNLWNSSIKKKLRQKGIDPNTHKPLLVEVERSKAAPTTSTERTSESSDVDPSSGGALGNSSHLLLSETAQSPQLPALGKHRRETTSLAHLRVPPKELFLDQLVSGHENLTGCRTAGPVPNFPFQQLMCYSNEFGNKNGASNNSLWFNHNETSGSTISTVMPPVSPSTLSTSTGLNRSPDNPHSGGTGIQSTQFYWDTANPSSCSSRGSSGSNGLGFELQSTSSLLESSVFPWTELTPDKNSQVHLGEELKWPDLLHGTFTDTPATMQNLSQSLYEDVIKAESQFNIEGLCAAWSQNLQPQQHLQVSDLYDKDLQRMSLSFENI
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGGAGGCACTCCTGCTGTTACAAGCAGAAGCTGAGGAAGGGGCTCTGGTCTCCTGAGGAAGATGAGAAGCTCATGAACCACATAACCAAGCACGGGCATGGCTGCTGGAGCACTGTCCCCAAGCTTGCAGGGCTTCAGAGGTGTGGCAAGAGCTGCAGGCTGAGGTGGATAAATTACCTGAGGCCTGACCTTAAAAGAGGCGCATTCTCTGAGGAGGAGGAAGACCTCATCGTCGAACTTCATGCTGTACTGGGGAACAGGTGGTCTCAGATTGCAACAAGGTTGCCTGGAAGAACTGACAACGAGATTAAGAATCTCTGGAACTCGAGCATCAAGAAGAAGCTCCGGCAGAAAGGCATTGACCCCAACACCCACAAGCCGCTCCTTGTCGAGGTTGAACGCAGCAAAGCAGCTCCAACGACCAGTACCGAGAGGACCTCTGAGTCCAGTGATGTTGACCCTTCAAGTGGCGGCGCACTTGGCAACTCGAGCCATCTTCTTCTCAGCGAGACAGCACAATCACCACAGCTGCCAGCGCTCGGTAAGCATCGCAGAGAAACTACTAGCCTCGCACATCTAAGGGTGCCGCCCAAGGAGTTGTTCCTTGACCAGCTGGTTTCTGGTCATGAGAACCTCACTGGCTGCCGCACAGCAGGCCCAGTTCCAAATTTCCCTTTCCAACAGCTCATGTGCTACAGCAATGAATTTGGCAACAAGAATGGTGCTAGTAACAATTCACTCTGGTTTAACCACAATGAGACAAGTGGCAGCACCATTTCCACTGTGATGCCACCAGTTTCGCCATCAACTCTCTCAACATCAACAGGGCTCAATAGGTCACCAGACAACCCACACTCGGGAGGCACTGGTATTCAGAGCACCCAGTTCTACTGGGACACTGCTAATCCTAGCAGCTGCAGCAGTAGAGGAAGCAGCGGAAGCAACGGCTTGGGATTTGAGCTGCAAAGCACAAGCTCGCTTCTGGAGAGTAGTGTTTTCCCATGGACAGAATTAACACCAGACAAAAATAGCCAAGTTCACCTTGGGGAAGAACTCAAGTGGCCCGACTTGCTCCACGGAACATTCACGGACACACCTGCAACCATGCAGAATCTTAGCCAGTCACTGTACGAAGATGTTATCAAAGCCGAGAGCCAATTCAACATAGAGGGCCTCTGTGCAGCCTGGTCTCAAAATCTGCAGCCACAGCAACATCTGCAGGTATCAGATTTGTATGACAAGGATTTGCAAAGGATGTCCTTGTCTTTTGAGAATATC
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