TFome clone pUT3447

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B302_F
Temperature (ºC)	64.7
Sequence Copy to clipboard	caccATGGACATGGACATGGC

3' Primer

Name	B302_R
Temperature (ºC)
Sequence Copy to clipboard	GCAAAGGTCATCCAGGCTCCACA

PCR Condition

5 l GC buffer (NEB) 1 l primer forward 20mM 1 l pr

Amino Acid Sequence

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MDMDMAMMGFDEDLDQVLAAMGPADSSATSSAPNAAAPAPLAVVPAGSSDDEEAAAADSADDLRRGPWTVDEDILLVNYIAAHGEGRWNSLARSAGLKRTGKSCRLRWLNYLRPNVRRGNITAEEQLLILDLHSRWGNRWSKIAQQLPGRTDNEIKNYWRTRVQKHARHLRCDVNSAHFRHIVRHVWMPRLRERAQAGGVQAPATTTASAPPACYNYCGSQHLVQQQGAHRGVTAASEAHHHHQYYSEPAGPTSAATALSPDDASSALRSSSLRADDASHYTAAYTYTSAATATPTTNDQGYEQTTTDDDVFAGSTTSELLVTATGGPDDEYSSMIGLQLQDFGFEDFEYGLWSLDDLC

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGACATGGACATGGCCATGATGGGCTTCGATGAGGACCTAGACCAGGTGCTGGCGGCGATGGGCCCCGCCGACAGCTCGGCGACGTCGTCTGCGCCCAACGCCGCCGCACCAGCACCACTAGCTGTCGTCCCAGCAGGCAGCAGCGACGATGAGGAGGCCGCCGCCGCGGATAGCGCCGACGACCTCCGGAGGGGCCCCTGGACGGTGGACGAGGACATCCTCCTCGTCAACTACATCGCCGCCCACGGCGAGGGCCGCTGGAACTCGCTCGCCCGCTCCGCGGGCCTGAAACGCACCGGCAAGAGCTGCCGCCTGCGGTGGCTGAACTACCTGCGGCCCAACGTGCGGCGGGGCAACATAACCGCCGAGGAGCAGCTGCTGATCCTGGACCTGCACTCGCGCTGGGGCAACAGGTGGTCCAAGATCGCGCAGCAGCTCCCCGGGCGCACCGACAACGAGATCAAGAACTACTGGCGCACCCGCGTGCAGAAGCACGCCAGGCACCTCCGCTGCGACGTCAACAGCGCCCACTTCCGCCACATCGTGCGCCACGTCTGGATGCCTCGCCTGCGAGAGCGCGCCCAGGCCGGCGGCGTACAGGCTCCAGCTACCACCACGGCCAGCGCGCCGCCCGCGTGTTATAACTACTGCGGGAGCCAACACCTGGTGCAGCAGCAAGGCGCCCACCGCGGCGTGACGGCGGCGAGCGAAGCTCACCACCACCACCAATACTACAGCGAGCCGGCAGGCCCGACGTCGGCGGCCACGGCCCTCAGCCCCGACGACGCCTCCAGCGCGCTGCGGTCGTCGTCGCTCAGGGCGGACGACGCGTCGCATTACACTGCAGCTTACACTTACACCTCGGCGGCCACGGCGACCCCGACGACGAACGACCAAGGCTACGAACAAACTACGACTGACGACGACGTGTTCGCCGGGTCGACCACATCGGAGCTCCTCGTCACCGCTACCGGCGGCCCTGACGACGAGTACAGCTCCATGATCGGCCTGCAGCTGCAGGACTTTGGGTTTGAGGATTTCGAGTACGGCCTGTGGAGCCTGGATGACCTTTGC