| TFome pUT3460 | |
|---|---|
| Template | BT042226.1 |
| Genbank Insert ID | KJ726915 |
| Protein Name | ZmHB21 |
| Species | Zea mays |
| TF Family | Homeobox |
| Gene id | GRMZM2G076272 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT3460
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B509_F |
| Temperature (ºC) | 69.7 |
| Sequence
|
caccATGACCGGCAACGCGTCTTATC |
3' Primer
| Name | B509_R |
| Temperature (ºC) | |
| Sequence
|
TCGGCTTCCACAGCCGAACG |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f
Amino Acid Sequence
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MTGNASYQHLGLGVDATMRSCFASGDGSVISSADAPFFHVPEYTLHGGGGFGFGEPAADVAASSFLADGSVLLAGQLLRATALQSVSPEERHGAYYGVTGGSSSYGPSPWDVTVAHAPSRMTKQPVAGEPEGGWIHESSYYCPATWFSGDGFRDPFAGAAASELSLRLRAGSSPTAGAASVSLPDQSSEVSCSGLTHWSSGGGPGMFQLPCGGAGQVAVAARPGPMHFSQVLSRWSGYADVTQQVLDEFVIRLLQDVAGFAGGGEASCPLPSSSYCSKTTSSNPNPSVFVSSEEHHNELKNDFQKLLQIMDQRCKQCLDEIQNAASKYGSLVRPGGGGGALSTLFAQRAVSATHRRLRARITGEIAAATRGGNQPSSSSSLSLADRERSWESAFIQKHWALRQLRRGDQQSWRPQRGLPEKSVAVLKAWMFENFLRPYPRDKEKEMLAARSGLSRSQVQSPTGSSTPAFGCGSR
TFome clones do not contain stop codon at 3'end.
CDS
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ATGACCGGCAACGCGTCTTATCAGCATCTGGGACTGGGAGTCGACGCCACGATGCGCAGCTGCTTCGCCAGCGGCGACGGCAGTGTTATCAGCAGCGCCGACGCGCCCTTCTTCCACGTCCCGGAGTACACACTGCACGGCGGCGGCGGCTTTGGGTTTGGCGAACCCGCAGCGGACGTCGCCGCCTCGAGCTTCTTGGCGGATGGTTCGGTGCTGCTCGCCGGCCAGCTGCTCCGTGCCACCGCACTGCAGAGCGTGTCGCCGGAGGAGAGGCACGGTGCCTACTACGGCGTCACGGGCGGGTCGTCGTCGTACGGCCCGTCGCCCTGGGATGTCACCGTTGCTCACGCGCCCAGCAGGATGACCAAGCAGCCTGTCGCTGGAGAGCCGGAAGGCGGCTGGATCCACGAGTCTTCGTACTACTGCCCGGCGACGTGGTTCTCTGGCGACGGCTTCCGGGACCCGTTCGCCGGGGCGGCGGCGAGCGAGTTGTCGCTCAGGCTGCGCGCTGGGTCCTCCCCGACGGCAGGTGCGGCGAGCGTGAGCCTGCCGGATCAGTCCTCGGAGGTGAGCTGCTCCGGCTTGACGCATTGGAGTAGCGGCGGCGGCCCCGGCATGTTCCAGCTTCCTTGCGGCGGCGCCGGACAGGTGGCGGTGGCGGCACGTCCGGGCCCCATGCACTTCTCGCAGGTCCTGTCGCGGTGGTCCGGGTACGCGGACGTCACGCAGCAGGTGTTGGACGAGTTCGTCATCCGCCTGCTGCAAGACGTGGCCGGGTTCGCCGGCGGCGGTGAGGCGAGCTGCCCGCTGCCATCGTCAAGTTATTGCTCCAAGACGACGTCATCCAACCCGAACCCGTCGGTGTTTGTTAGCTCGGAAGAGCACCACAATGAGCTCAAGAACGACTTCCAGAAACTGCTACAGATAATGGACCAACGGTGCAAGCAATGCCTGGACGAGATCCAGAATGCGGCGTCGAAGTACGGCAGCCTTGTGCGCCCGGGCGGCGGCGGCGGCGCCCTCTCGACGCTGTTTGCGCAGCGCGCGGTGTCAGCGACGCACCGGAGGCTCAGGGCGCGGATCACCGGCGAGATCGCGGCGGCCACGCGGGGAGGTAATCAGCCGTCGTCGTCGTCGTCGCTGTCGCTGGCCGACAGGGAGCGCAGCTGGGAGTCGGCCTTCATCCAGAAGCACTGGGCGCTGCGGCAGCTCAGGCGCGGCGACCAGCAATCGTGGCGCCCGCAGCGCGGCCTGCCGGAGAAGTCCGTGGCCGTGCTCAAAGCCTGGATGTTCGAGAACTTCCTCCGCCCGTACCCGAGAGACAAGGAGAAGGAGATGTTAGCAGCCAGGAGCGGACTGAGCAGGAGCCAGGTACAGTCTCCAACTGGTTCATCAACGCCCGCGTTCGGCTGTGGAAGCCGA
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