| TFome pUT3470 | |
|---|---|
| Template | BT033360.1 |
| Genbank Insert ID | KJ726925 |
| Protein Name | ZmMADS6 |
| Species | Zea mays |
| TF Family | MADS |
| Gene id | GRMZM2G159397 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.T666C p.(=). In template FlcDNA. |
| Request Information | Deposited to ABRC |
TFome clone pUT3470
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B612_F |
| Temperature (ºC) | 73.5 |
| Sequence
|
caccATGGGGAGGGGCCGGGTC |
3' Primer
| Name | B612_R |
| Temperature (ºC) | |
| Sequence
|
TGGCAACCACGGGGGCAGGA |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f
Amino Acid Sequence
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MGRGRVELKRIENKINRQVTFAKRRNGLLKKAYELSVLCDAEVALIIFSNRGKLYEFSSTQSMPKTLEKYQKCSFAGPETALQNRENEQLKSSRNEYLKLKARVDNLQRTQRNLLGEDLESLGIKELEHLEKQLDSSLKHIRSTRTQHMVDQLTELQKKEQMFCEANKCLRRRLEESNQVIWQHAWEQSERHSEVQPQQLNGNNFFHPLDGAGEPTLQIGYPSEALTSSCMTTFLPPWLP
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGGAGGGGCCGGGTCGAGCTCAAGCGGATCGAGAACAAGATCAACCGCCAGGTCACCTTCGCCAAGCGCCGCAACGGCCTGCTCAAGAAGGCGTACGAGCTCTCGGTGCTCTGCGACGCCGAGGTCGCGCTCATCATCTTCTCCAACCGCGGCAAGCTCTACGAGTTCTCCAGCACGCAGAGCATGCCAAAAACACTTGAAAAGTACCAAAAATGCAGTTTTGCAGGGCCTGAAACAGCACTCCAGAATAGAGAGAATGAGCAACTGAAAAGTAGCCGCAATGAGTACCTCAAACTGAAGGCACGTGTTGATAATTTACAGCGGACTCAAAGGAACTTGCTTGGTGAAGATCTTGAGTCATTAGGCATAAAAGAGCTGGAGCACCTGGAGAAGCAGCTCGATTCGTCCTTGAAGCACATAAGATCTACAAGGACACAACACATGGTTGATCAACTGACAGAACTTCAGAAAAAAGAACAAATGTTTTGTGAAGCAAATAAGTGTCTTCGAAGAAGACTGGAGGAGAGCAACCAGGTTATATGGCAGCATGCGTGGGAGCAAAGCGAGCGGCATTCTGAAGTGCAGCCGCAGCAGCTCAATGGCAATAACTTCTTCCATCCCCTCGATGGTGCTGGTGAACCCACCCTTCAGATAGGGTATCCCTCAGAGGCACTGACTAGCTCATGCATGACCACCTTCCTGCCCCCGTGGTTGCCA
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