| TFome pUT3645 | |
|---|---|
| Template | BT083691 |
| Genbank Insert ID | KJ726951 |
| Protein Name | ZmTCP12 |
| Species | Zea mays |
| TF Family | TCP |
| Gene id | GRMZM2G093895 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C663T p.(=) Not present in template FlcDNA. |
| Request Information | Deposited to ABRC |
TFome clone pUT3645
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT2554up |
| Temperature (ºC) | 49.6 |
| Sequence
|
caccATGGACCATGGCGGC |
3' Primer
| Name | UT2554dn |
| Temperature (ºC) | |
| Sequence
|
CTGTGAGCTGTTGTTGCC |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MDHGGGGGGGGAPSSSNSAGGAAAGGGATAGGASGGRGSEHHPFYYAGPAASNSGVPQAQQQPSPFAGALAIAPVPEQPQPSSADKKALVPAAPAAAGPVVAKRPSKDRHTKVDGRGRRIRMPALCAARVFQLTRELGHKSDGETIEWLLQQAEPAILAATGTGTIPANYSSLNISIRSGAAAPAARAAPFPALALHPHHHHQAAPAHDMSAMMAGYHHHLLPPQPQPQEDPSAGDAYMRKRYREDLFKEDDERQDPSAPKAREQQAATPPPQPQPSATMWAAAAPSGGFWMLPVSASSAAAARPAEQPMWSFSGGGGTGTVQAPLQFMSRASYPGGAAGGGGGAMSDTNLGMLAALNAYNRGGSEEQQQTEGEQQHGGDGGVNDDGDEDGDDSAEENNGNNSSQ
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACCATGGCGGCGGGGGAGGAGGGGGTGGGGCGCCGTCGAGCAGCAACAGCGCCGGAGGGGCCGCCGCCGGCGGCGGCGCCACCGCTGGCGGCGCGAGCGGCGGTCGCGGTAGCGAGCACCACCCGTTCTACTACGCCGGGCCCGCGGCGTCCAACTCCGGAGTGCCACAGGCCCAGCAGCAGCCGTCGCCGTTCGCTGGCGCGCTCGCTATCGCGCCCGTGCCCGAGCAGCCGCAGCCGTCGTCCGCCGACAAGAAGGCGCTCGTGCCTGCGGCCCCCGCGGCGGCGGGGCCCGTAGTAGCCAAGCGGCCGTCCAAGGACCGGCACACCAAGGTGGACGGGCGGGGGCGGCGGATTCGGATGCCGGCGCTGTGCGCGGCGCGGGTGTTCCAGCTGACCCGCGAGCTCGGCCACAAGTCCGACGGCGAGACCATCGAGTGGCTGCTGCAGCAGGCGGAGCCGGCCATCCTGGCGGCTACCGGCACCGGCACCATCCCGGCCAACTACTCCTCGCTTAACATCTCCATCCGCTCCGGCGCCGCCGCCCCCGCCGCCCGCGCCGCGCCGTTCCCGGCGCTCGCGCTCCACCCGCACCACCACCACCAGGCGGCCCCCGCGCACGACATGTCCGCCATGATGGCTGGGTACCATCACCACCTCCTCCCGCCGCAGCCGCAGCCGCAGGAGGACCCCAGCGCCGGCGACGCCTACATGCGGAAGCGCTACCGGGAGGACCTGTTCAAGGAGGACGACGAGCGCCAGGATCCGAGCGCGCCCAAAGCGCGCGAGCAGCAGGCGGCGACTCCGCCGCCGCAGCCGCAGCCTTCCGCCACAATGTGGGCCGCCGCCGCCCCGTCCGGCGGGTTCTGGATGCTGCCCGTGTCCGCGAGCTCGGCCGCCGCGGCGCGGCCGGCCGAACAGCCCATGTGGTCCTTCAGCGGCGGCGGCGGCACGGGCACCGTGCAGGCCCCGCTCCAGTTCATGTCCAGGGCCAGCTACCCCGGTGGCGCTGCAGGCGGCGGAGGCGGAGCCATGTCCGACACCAACCTTGGCATGCTCGCCGCGCTGAACGCGTACAACCGTGGCGGTAGCGAGGAGCAGCAGCAGACCGAGGGGGAGCAGCAGCACGGCGGCGACGGCGGGGTTAACGATGACGGAGACGAAGATGGTGACGACAGCGCTGAAGAGAACAATGGCAACAACAGCTCACAG
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