TFome clone pUT3673

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	Tup4
Temperature (ºC)	58.9
Sequence Copy to clipboard	caccATGGACATGGAGGAACAGTTGCTG

3' Primer

Name	Tdn4
Temperature (ºC)
Sequence Copy to clipboard	AATCAAACAATCAATCGCTGTTACAAGC

PCR Condition

Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&

Amino Acid Sequence

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MDMEEQLLHLEHFMPSSPGFFDVGSGHDPAHFPNGGLFVEQVGATDDGGWVEELMQLGEELFGGPGDGGIMGDADDHYQQWQCDDDVDGGSPDGGPPPSVSLDGDDASPGEQGAGELASEPHREDGDDVSGATRKRRDRSKTIVSERKRRVRMKEKLYELRSLVPNITKVRLAPVWTSEFGRLFAGELGHSIHVVPLHACARHVQMDKASIIADAVVYVKNLQAHARKLKDEVATLEARPRSPTRQDQPQLQHGRSAGPGRRRQQQQQVERRDAGSAGAGARVTHVGAAQVGDGRFFVTVECERRDGVAAPLCAAAESLLCFRVESSSIGRSGPDRVVSTLTLKVWTGLDWTGSMGWLVTAIDCLI

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGACATGGAGGAACAGTTGCTGCACCTGGAGCACTTCATGCCCAGCAGCCCGGGCTTCTTTGACGTCGGCAGTGGCCACGACCCCGCCCACTTCCCGAACGGCGGGTTATTCGTCGAGCAGGTCGGCGCCACCGACGACGGCGGGTGGGTGGAGGAACTCATGCAGCTGGGCGAGGAGCTGTTCGGCGGTCCGGGCGATGGCGGCATCATGGGCGACGCCGACGACCACTACCAGCAGTGGCAGTGCGACGACGACGTCGACGGCGGCTCCCCAGACGGCGGTCCGCCACCGAGCGTGTCCTTGGACGGCGACGACGCGAGCCCAGGGGAGCAAGGCGCCGGGGAGCTCGCCTCCGAGCCGCACCGCGAGGACGGCGACGACGTGTCGGGCGCGACGCGGAAGCGGAGGGACCGGTCCAAGACCATCGTGTCGGAGCGGAAGCGGAGGGTCCGGATGAAGGAGAAGCTCTACGAGCTCAGGTCGCTCGTCCCCAACATCACAAAGGTGAGGCTAGCTCCGGTCTGGACTTCGGAGTTCGGACGACTGTTCGCTGGGGAGCTCGGTCATTCGATTCATGTCGTCCCCCTGCATGCATGTGCGCGCCATGTGCAGATGGACAAGGCGTCCATCATCGCCGACGCGGTGGTGTACGTGAAGAACCTCCAGGCGCACGCCAGGAAGCTGAAGGACGAGGTCGCCACGCTGGAGGCCCGGCCGAGATCGCCTACCCGACAGGACCAGCCGCAGCTGCAGCACGGCCGGAGCGCGGGGCCCGGCCGACGACGCCAGCAGCAGCAGCAGGTGGAGCGCCGCGACGCGGGGAGCGCGGGCGCCGGCGCCCGGGTGACGCACGTGGGCGCGGCGCAGGTCGGCGACGGCAGGTTCTTCGTGACCGTGGAGTGCGAGCGGCGGGACGGCGTGGCGGCGCCGCTCTGCGCGGCCGCCGAGTCGCTGCTGTGCTTCCGGGTGGAGAGCTCCAGCATCGGCCGGTCGGGGCCGGACCGCGTCGTGTCCACGCTCACGCTCAAGGTCTGGACTGGACTGGACTGGACTGGATCCATGGGGTGGCTTGTAACAGCGATTGATTGTTTGATT