| TFome pUT3685 | |
|---|---|
| Template | DT647742 |
| Genbank Insert ID | KJ726984 |
| Protein Name | ZmC3H48 |
| Species | Zea mays |
| TF Family | C3H |
| Gene id | GRMZM2G044004 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT3685
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT-2581up |
| Temperature (ºC) | 65.3 |
| Sequence
|
caccATGGACGCCTGGGAGGC |
3' Primer
| Name | UT-2581dn |
| Temperature (ºC) | |
| Sequence
|
CAGCCCAATGGCTTCATGC |
PCR Condition
Amino Acid Sequence
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MDAWEATKAVFDRVRALDPDNASKIMGLLLIQDNSDKELIRLAFGPDHLLHAFVSAARADLAAKPASPPSPVLGPLHQTWGAPPHPSPTAGSDHQHQAPFAADLALGYDFDGAGAGADAFFPDDYDCWSPAGAAHRRSFSLSDAEATAAAAGGAAWRPCMYFARGFCKNGSSCRFLHGFPEDDDAAAEREMVVMRAKALAAAAARPQQQQQLMASAFPFSPSPPKGVNLNFLLHQHQQQNEPQRAAAAAAMLLQGGDDMHSRFPVRSPRMDRGELMSSPAARQIYLTFPADSTFSEEDVSNYFSMYGPVQDVRIPYQQKRMFGFVTFVYAETVKIILSKGNPHFVCDARVLVKPYKEKGKVPDRFRKLQHPHHGDFAGCTSPTGLLDSRDPFDLQQPQIGPRMMYGNVAGHEAFLRRKLEEQQQAAELQQAIELEGRRFMGLHLLDLKSRGHHHLGSSPAAMATTTLGQGDDGCKGRSANGNDNAVAFHLEDANIQDTPTKMNSNSLAMSGGPAAGAGGEHEEQQQDGDGDGSPKQAVNPGKEEKTESGPVTATPIVACGFQESGVVEHILPDSPFASPSKASTQNGSIISNAASSLFPPPASTMELPPYSSCFFQAPRFSPGHEAIGL
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACGCCTGGGAGGCCACCAAGGCGGTGTTCGACCGGGTGCGGGCGCTGGACCCGGACAACGCGTCCAAGATCATGGGCCTGCTGCTCATCCAGGACAACAGCGACAAGGAGCTCATCCGCCTCGCCTTCGGCCCCGACCACCTCCTCCACGCCTTCGTCTCCGCCGCGCGCGCCGACCTCGCCGCCAAGCCCGCGTCCCCGCCGTCGCCCGTGCTGGGCCCGCTCCACCAGACCTGGGGAGCGCCGCCGCACCCGAGCCCTACCGCTGGCTCCGACCACCAACACCAGGCCCCCTTCGCCGCCGACCTCGCGCTCGGCTACGACTTCGACGGCGCCGGCGCCGGCGCTGACGCGTTCTTCCCCGACGACTACGACTGCTGGTCACCGGCCGGCGCCGCCCACCGCCGCAGCTTCTCGCTCAGCGACGCCGAGGCCACCGCCGCCGCCGCCGGAGGCGCTGCGTGGAGGCCATGCATGTACTTCGCGCGCGGGTTCTGCAAGAACGGCTCCTCCTGCCGCTTCCTCCACGGCTTTCCGGAGGACGATGACGCCGCCGCGGAGCGCGAGATGGTCGTCATGCGCGCCAAAGCCCTTGCCGCCGCCGCCGCGCGCCCCCAGCAGCAGCAGCAGCTCATGGCGTCCGCGTTTCCCTTCTCGCCGTCGCCGCCCAAGGGCGTCAACCTCAACTTCCTGCTCCACCAGCACCAGCAGCAGAACGAGCCGCAAAGGGCGGCGGCGGCGGCGGCCATGCTGCTCCAGGGCGGCGACGACATGCACAGCAGGTTCCCGGTGCGGTCGCCCCGGATGGACCGCGGCGAGCTCATGTCCAGCCCCGCCGCGCGGCAGATCTACCTCACCTTCCCGGCCGACTCCACCTTCAGCGAGGAGGACGTCTCCAACTACTTCAGCATGTACGGGCCGGTGCAGGACGTGCGCATCCCGTACCAGCAGAAGCGCATGTTCGGCTTCGTCACCTTCGTCTACGCCGAGACGGTGAAGATCATCCTCAGCAAGGGCAACCCGCACTTCGTTTGCGACGCTCGCGTGCTCGTCAAGCCCTACAAGGAGAAGGGCAAGGTCCCCGACAGGTTCAGGAAGCTGCAGCACCCGCACCACGGCGACTTCGCCGGCTGCACGTCGCCCACCGGACTGCTGGATTCCAGGGACCCCTTCGACCTGCAGCAACCGCAGATTGGACCTAGGATGATGTACGGGAACGTTGCTGGCCACGAAGCATTCCTGAGGAGGAAACTGGAAGAGCAGCAGCAGGCGGCCGAGCTGCAGCAGGCCATCGAGCTGGAGGGGCGCCGCTTCATGGGGCTGCACCTCCTCGACCTCAAGAGCAGGGGCCACCACCACCTCGGCTCCTCCCCCGCGGCCATGGCCACCACCACTCTGGGGCAAGGCGACGACGGCTGCAAAGGCCGCAGCGCCAACGGGAATGACAATGCCGTCGCCTTCCATTTGGAGGATGCCAACATCCAAGATACTCCTACCAAGATGAACAGTAACAGCCTTGCAATGAGTGGTGGGCCTGCAGCCGGTGCAGGAGGCGAGCACGAGGAGCAGCAGCAGGATGGGGACGGGGATGGCAGTCCCAAGCAGGCAGTCAACCCTGGGAAAGAGGAGAAGACGGAATCTGGTCCTGTCACAGCCACGCCCATTGTTGCCTGTGGATTCCAAGAAAGTGGCGTGGTGGAGCACATCTTGCCTGACAGCCCCTTTGCGTCCCCCAGCAAGGCCTCCACTCAAAATGGGAGCATCATCAGCAATGCGGCTTCATCCCTCTTCCCCCCGCCTGCATCCACCATGGAGCTGCCCCCGTACAGCTCTTGCTTCTTCCAGGCGCCCAGGTTTTCTCCTGGGCATGAAGCCATTGGGCTG
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