| TFome pUT5324 | |
|---|---|
| Template | BT038003 |
| Genbank Insert ID | KJ727466 |
| Protein Name | ZmMYBR22 |
| Species | Zea mays |
| TF Family | MYB-related |
| Gene id | GRMZM2G468479 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5324
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B867F |
| Temperature (ºC) | 59.9 |
| Sequence
|
caccATGCCAGTTAATTGGCTGCATAA |
3' Primer
| Name | B867R |
| Temperature (ºC) | |
| Sequence
|
CATGGGCGAGACAGGAGGGC |
PCR Condition
Step 1, 98°C 40 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MPVNWLHNRPDNMRRNYLSNDVYNILEMDHIPDLTPSENRNHAINGTTVFEELKSLRLTSDRQPKAESIIHSERPPYRSTSDGPTSSSGFSCPDNNAGHNGELESYVAAFFENSLSQARQMVDEAIQANSGGSDDEKVCMLNQVTGCLGSDSRGVQLPSDLISNCIATVISIKRLSDSRHPFGNVAGVLEHALSMLRPSCPQNLGVYTEIKRDLCTIANQIFALVPTASGNCSPPVSPM
TFome clones do not contain stop codon at 3'end.
CDS
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ATGCCAGTTAATTGGCTGCATAATCGTCCTGACAACATGAGAAGGAACTACCTTTCAAATGATGTGTACAACATACTGGAGATGGATCACATTCCTGACCTTACACCAAGTGAGAACCGGAATCATGCTATAAATGGAACTACTGTATTCGAGGAGCTCAAAAGTCTGCGCTTAACATCAGATAGACAGCCTAAGGCAGAATCTATTATACATAGTGAACGGCCACCATATAGGAGTACATCTGATGGCCCTACAAGTTCTAGTGGTTTCTCTTGCCCAGACAATAATGCTGGTCACAACGGTGAACTAGAGTCATATGTAGCTGCATTTTTTGAAAACTCACTATCACAAGCCAGGCAGATGGTTGACGAAGCCATCCAGGCAAACTCTGGTGGTAGTGATGATGAAAAGGTATGCATGTTAAATCAAGTGACAGGATGTCTAGGCTCAGATTCTCGTGGCGTGCAACTTCCATCTGACCTGATATCAAACTGCATCGCAACGGTTATTTCAATCAAGCGCCTTAGCGACTCGCGACATCCATTTGGTAACGTAGCAGGCGTCTTGGAGCATGCTTTATCAATGCTGCGTCCAAGCTGTCCACAGAACCTCGGAGTTTACACAGAAATAAAGAGAGACCTTTGCACCATAGCGAACCAGATTTTTGCACTTGTGCCAACAGCATCGGGCAATTGCAGCCCTCCTGTCTCGCCCATG
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