| TFome pUT5344 | |
|---|---|
| Template | BT040003 |
| Genbank Insert ID | KJ727485 |
| Protein Name | ZmEREB152 |
| Species | Zea mays |
| TF Family | AP2/ERF-AP2 |
| Gene id | GRMZM2G139082 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5344
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C458F |
| Temperature (ºC) | 61.2 |
| Sequence
|
caccATGGACATGGACATGAGCTCAG |
3' Primer
| Name | C458R |
| Temperature (ºC) | |
| Sequence
|
TGTCAAAGTTGGTGACGGCGTT |
PCR Condition
Step 1, 98°C 40 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MDMDMSSAYPHHWLSFSLSNNYHHGLLEAFSNSSGTPLGDEQGAVEESPRTVEDFLGGVGGAGAPPQPAAAADQDHQLVCGELGSITARFLRHYPAAPAGTTVENPGAVTVAAMSSTDVAGAESDQARRPAETFGQRTSIYRGVTRHRWTGRYEAHLWDNSCRREGQSRKGRQVYLGGYDKEEKAARAYDLAALKYWGPTTTTNFPVSNYEKELEEMKSMTRQEFIASLRRKSSGFSRGASIYRGVTRHHQHGRWQARIGRVAGNKDLYLGTFSTQEEAARRTTSLRSSSAGSTPSPTLT
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACATGGACATGAGCTCAGCTTATCCCCACCATTGGCTCTCCTTCTCCCTCTCCAACAACTACCACCATGGCCTACTCGAAGCCTTCTCTAACTCCTCCGGTACTCCTCTTGGAGACGAGCAGGGCGCAGTGGAGGAGTCCCCGAGGACGGTGGAGGACTTCCTCGGCGGCGTCGGTGGCGCCGGCGCCCCGCCGCAGCCGGCGGCGGCTGCAGATCAGGATCACCAGCTTGTGTGCGGCGAGCTGGGCAGCATCACAGCCAGGTTCTTGCGCCACTACCCGGCGGCGCCAGCTGGGACGACGGTGGAGAACCCCGGCGCGGTGACCGTGGCGGCCATGTCGTCGACGGACGTGGCCGGGGCGGAGTCCGACCAGGCGAGGCGGCCCGCCGAGACGTTCGGCCAGCGCACATCCATCTACCGTGGCGTCACCAGGCACCGGTGGACGGGGAGATATGAGGCGCACCTGTGGGACAACAGCTGCCGCCGGGAGGGCCAAAGCCGCAAAGGACGGCAAGTCTACCTAGGAGGCTATGACAAGGAGGAGAAGGCGGCTAGAGCTTACGACCTCGCCGCGCTCAAGTACTGGGGGCCTACAACCACGACCAACTTCCCGGTGTCCAACTACGAGAAGGAGCTGGAGGAGATGAAGTCCATGACGCGGCAGGAGTTCATCGCGTCGTTGCGCAGGAAGAGCAGCGGCTTCTCACGAGGCGCCTCCATCTACAGAGGAGTCACAAGGCATCATCAGCACGGCCGGTGGCAGGCGAGGATCGGCAGGGTGGCCGGAAACAAGGACCTGTACTTGGGCACTTTCAGTACTCAGGAAGAGGCGGCGAGGCGTACGACATCGCTGCGATCAAGTTCCGCGGGCTCAACGCCGTCACCAACTTTGACA
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