| TFome pUT5353 | |
|---|---|
| Template | BT063597.1 |
| Genbank Insert ID | KJ727494 |
| Protein Name | ZmMYB99 |
| Species | Zea mays |
| TF Family | MYB |
| Gene id | GRMZM2G175232 |
| transcript | T01 |
| Vector | pENTR/D-TOPO |
| Note | . c.C36T p.G12(=) c.G840A p.G240(=) c.G850A p.A284T Not present in template FlcDNA. Not in functional domain. |
| Request Information | Deposited to ABRC |
TFome clone pUT5353
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C148_F |
| Temperature (ºC) | 76.0 |
| Sequence
|
caccATGGGACGGCTCTCCTGC |
3' Primer
| Name | C148_R |
| Temperature (ºC) | |
| Sequence
|
GAAGTATTCCAAGCCGAAGTCGAG |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f
Amino Acid Sequence
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MGRLSCGGGGGGGVQPKLRKGLWSPEEDEKLYNHIIRHGVGCWSTVPKLAGLQRCGKSCRLRWINYLRPDLKRGSFSQQEEDLIVALHEILGNRWSQIASHLPGRTDNEIKNFWNSCLKKKLRQRGIDPATHKPVAAAEASAALPDARDHDREPGGAGAGADADGLAQQSKQPAAAVFDPFPVVDFGFDLSGGGGVAALYGGPYDAAAGKASADDGGFVADYSSVLDVSENLGYGESSSNSSNWGNGAEMGNAAAAVLDGEVLHWAKPEPYTELEQRSAGQAATDDDALHHRFLLPCGQEQSLAHLDFGLEYF
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGACGGCTCTCCTGCGGCGGCGGTGGCGGCGGTGGCGTGCAGCCCAAGCTCCGCAAGGGGCTCTGGTCGCCCGAGGAAGACGAGAAGCTGTACAATCACATCATCCGACACGGCGTCGGCTGCTGGAGCACTGTCCCCAAGCTCGCAGGGCTGCAGCGCTGCGGCAAGAGCTGCCGCCTGCGGTGGATCAACTACCTCCGCCCCGACCTCAAGCGCGGCAGCTTCTCGCAGCAGGAGGAGGACCTCATCGTCGCGCTCCACGAGATCCTGGGGAACAGGTGGTCGCAGATCGCGTCGCACTTGCCGGGGCGGACGGACAACGAGATCAAGAACTTCTGGAACAGCTGCCTGAAGAAGAAGCTCCGGCAGCGCGGCATCGACCCGGCCACGCACAAGCCAGTCGCCGCGGCGGAGGCGAGCGCGGCGCTGCCGGACGCTCGAGACCACGACCGTGAGCCCGGAGGAGCCGGCGCCGGCGCCGACGCCGACGGCCTGGCGCAGCAGAGCAAGCAGCCGGCGGCGGCCGTGTTCGACCCGTTCCCTGTGGTCGACTTCGGCTTCGACCTGAGCGGCGGCGGCGGCGTGGCGGCATTGTACGGCGGCCCGTACGACGCCGCCGCCGGCAAGGCGTCGGCGGACGACGGCGGGTTCGTGGCCGACTACAGCAGCGTGCTGGACGTGTCGGAGAACCTGGGATACGGGGAGAGCTCCAGCAACAGCAGCAACTGGGGGAACGGCGCGGAGATGGGCAATGCCGCCGCCGCCGTGCTCGACGGCGAGGTGCTGCACTGGGCCAAGCCGGAGCCGTACACGGAGCTGGAGCAGCGGAGCGCGGGACAAGCCGCCACGGACGACGACGCGCTGCATCATAGGTTCTTGCTGCCCTGCGGCCAGGAGCAGAGCCTGGCGCATCTCGACTTCGGCTTGGAATACTTC
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