| TFome pUT5354 | |
|---|---|
| Template | BT087447.1 |
| Genbank Insert ID | KJ727495 |
| Protein Name | ZmMYB139 |
| Species | Zea mays |
| TF Family | MYB |
| Gene id | GRMZM2G087955 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT5354
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B288_F |
| Temperature (ºC) | 70.6 |
| Sequence
|
caccATGGCGGCGTCGCCTTCCCC |
3' Primer
| Name | B288_R |
| Temperature (ºC) | |
| Sequence
|
GTCGATCTCGGCCATGCCCATCAC |
PCR Condition
40 sec 98°C, 30x(15sec 98°C, 30sec 58°
Amino Acid Sequence
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MAASPSPSGSGGGKAGVDRIKGPWSPEEDEALQRLVRRHGARNWSLISRSVPGRSGKSCRLRWCNQLSPRVEHRPFTPDEDDAILRAHARFGNKWATIARLLSGRTDNAIKNHWNSTLKRERERKREYYADARPGPGDQRSLKRTSSDGPPGSPSGSDLSDSSHQSLPTVMPSAASTAVTSQQQLQHVYYRPVPRAGGVVLLPVAPPSPQAPPATSLSLSLSLPGLDQQPEPSPPPPPTPQAQASSPLSAEFLLMMQEMIRIEVRNYMSGSGFDAHAVMGMAEID
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCGGCGTCGCCTTCCCCGTCCGGCAGCGGCGGCGGCAAGGCCGGCGTGGACCGGATCAAGGGCCCCTGGAGCCCCGAGGAGGACGAGGCCCTGCAGCGCCTCGTGCGCCGCCACGGCGCGCGCAACTGGTCCCTCATCAGCCGCTCCGTCCCGGGCCGGTCCGGCAAGTCGTGCCGCCTCCGGTGGTGCAACCAGCTGTCGCCGCGGGTGGAGCACCGCCCCTTCACGCCCGACGAGGACGACGCCATCCTCCGCGCGCACGCCAGGTTCGGCAACAAGTGGGCCACCATCGCGCGCCTCCTCTCCGGACGCACGGACAACGCCATCAAGAACCACTGGAACTCCACGCTCAAGCGCGAGCGCGAGCGCAAGCGCGAGTACTACGCCGATGCGCGCCCCGGCCCCGGCGACCAGCGCTCGCTCAAGCGCACCAGCAGCGACGGGCCCCCCGGAAGCCCGTCCGGGTCCGACCTCAGCGACTCCAGCCACCAGAGCCTTCCCACCGTGATGCCGTCAGCCGCTTCTACCGCCGTCACCTCCCAGCAGCAGCTACAACACGTGTACTACCGCCCCGTGCCGCGCGCGGGCGGCGTGGTCCTGCTCCCGGTGGCGCCGCCTTCTCCGCAGGCTCCTCCAGCAACCTCCTTGTCGTTGTCCCTCTCCCTGCCAGGGCTGGACCAGCAGCCCGAACCATCCCCGCCGCCGCCGCCAACACCTCAAGCACAAGCAAGCTCGCCGTTGAGCGCGGAGTTCCTGTTGATGATGCAGGAGATGATCCGGATCGAGGTCCGGAACTACATGTCCGGGTCGGGCTTCGACGCACACGCCGTGATGGGCATGGCCGAGATCGAC
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