| TFome pUT5355 | |
|---|---|
| Template | BT036535.2 |
| Genbank Insert ID | KJ727496 |
| Protein Name | ZmMYB156 |
| Species | Zea mays |
| TF Family | MYB |
| Gene id | GRMZM2G147698 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.G222T p.E74D Not present in template FlcDNA, present in functional domain. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5355
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B294_F |
| Temperature (ºC) | 61.2 |
| Sequence
|
caccATGGCGAAGCAGTCGTG |
3' Primer
| Name | B294_R |
| Temperature (ºC) | |
| Sequence
|
GCACCAGTTGCTTAGCCC |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f
Amino Acid Sequence
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MAKQSCCHKKKLRRGLWSPEEDEKLMNHIAKYGHGCWSSVPKLAGLDRCGKSCRLRWINYLRPDLKRGTFSQEDEDLIIHLHSLLGNKWSQIAAQLPGRTDNEVKNFWNSYIKKRLRERGIDPATHQPLAEPAAAATATTTTTVSRRAVFGDVDLIPTTTTTTIQVPPPSFEGGPMLESVKLAVELDWPVAGDGVTSRPCYLQGGCFDMDALQQHCGSIPPVPVVPSASASSSTLTSMAEAEHCSTNNVAAGGNLPWLELGANAVADAGGHVVGSCYAGALDELRWSEYFDSAFQAAESQQGALQPGQCVYGGKDDVPVHFDVHGGLSNWC
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCGAAGCAGTCGTGCTGCCACAAGAAGAAGCTTAGGAGAGGGCTCTGGTCCCCCGAGGAGGACGAGAAGCTCATGAACCACATTGCCAAGTACGGCCACGGCTGCTGGAGCTCCGTACCCAAGCTTGCAGGCCTCGACAGGTGTGGCAAGAGCTGCAGGCTGAGGTGGATAAACTACCTGAGGCCAGACCTCAAGAGGGGCACATTCTCGCAAGAGGATGAGGACCTCATCATACACCTCCACTCCTTGCTGGGAAACAAGTGGTCTCAGATTGCGGCCCAGCTGCCTGGCCGGACGGACAACGAGGTCAAGAACTTCTGGAACTCGTACATCAAGAAGAGGCTCAGGGAGCGCGGCATCGACCCCGCCACCCACCAGCCGCTCGCCGAGCCTGCCGCCGCAGCCACTGCCACCACCACCACCACGGTCTCCCGCCGCGCGGTGTTTGGGGACGTCGACCTCATCCCGACTACTACTACTACTACAATCCAGGTGCCGCCGCCGTCGTTCGAGGGCGGCCCAATGCTGGAGAGCGTGAAGCTAGCGGTGGAGCTGGACTGGCCCGTCGCTGGCGACGGGGTAACGTCAAGGCCATGCTACCTGCAGGGGGGCTGCTTCGACATGGACGCGCTGCAGCAGCACTGCGGCTCCATCCCGCCGGTGCCGGTCGTCCCGTCGGCCTCCGCCAGCTCCAGCACGCTGACCTCGATGGCGGAAGCGGAACACTGCAGCACCAACAACGTCGCCGCCGGCGGCAACCTCCCATGGCTGGAGCTCGGGGCAAACGCGGTGGCTGACGCCGGCGGCCACGTCGTCGGCAGCTGCTACGCCGGCGCGCTGGACGAGCTCAGGTGGTCGGAGTACTTCGACAGTGCCTTCCAGGCCGCCGAGAGCCAGCAAGGCGCCCTGCAGCCGGGGCAGTGTGTCTACGGCGGCAAGGATGACGTCCCGGTGCACTTCGACGTCCATGGCGGGCTAAGCAACTGGTGC
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