| TFome pUT5361 | |
|---|---|
| Template | BT038988.1 |
| Genbank Insert ID | KJ727502 |
| Protein Name | ZmEREB34 |
| Species | Zea mays |
| TF Family | AP2/ERF-AP2 |
| Gene id | GRMZM5G846057 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT5361
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B400_F |
| Temperature (ºC) | 65.1 |
| Sequence
|
caccATGGACGCGAGCCTCCG |
3' Primer
| Name | B400_R |
| Temperature (ºC) | |
| Sequence
|
GAAGTTAAGCATCTCCCAGATGAGCTCC |
PCR Condition
PCR Reaction Mix:5 l GC buffer (NEB) 1 l primer fo
Amino Acid Sequence
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MDASLRTLPPAGTSSFPGEVRSAVSSLLLSSPGTSALDTVFSHLPPPVTIPPLGSSVYYRQCELLRHFAASQSQPQARQHAAACSASSSSPSSAAASSFQFQQSVPPDDMLRQKLYRGVRQRQWGKWVAEIRLPQNRVRVWLGTYDSPETAAHAYDRAAHRLRGEYARLNFPGAMDGPDPDCPEHLRHLRAAVDAKIQAIRARMARKRARARRQREERVSARSGLQAASEGAATTTTTTTTSETSAATPCGSPDGVLSVSAASGVADCPLERMPSFDPELIWEMLNF
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACGCGAGCCTCCGCACGCTGCCCCCGGCCGGCACCAGCAGCTTCCCGGGCGAGGTCCGCTCCGCCGTGTCCTCGCTGCTGCTCTCCTCCCCGGGCACCAGCGCCCTCGACACCGTGTTCTCGCACCTCCCGCCGCCGGTCACCATCCCGCCGCTCGGCTCCAGCGTCTACTACCGCCAGTGCGAGCTGCTCCGCCACTTCGCCGCCTCACAGTCACAGCCGCAGGCGCGCCAGCACGCCGCCGCCTGCTCGGCCTCCTCCTCGTCGCCGTCCTCCGCCGCCGCCTCCAGCTTCCAGTTCCAGCAGTCCGTGCCGCCCGACGACATGCTGCGGCAGAAGCTGTACCGCGGCGTGCGGCAGCGGCAGTGGGGCAAGTGGGTGGCGGAGATCCGGCTGCCGCAGAACCGGGTGCGCGTGTGGCTCGGCACCTACGACTCCCCCGAGACCGCCGCGCACGCCTACGACCGCGCCGCGCACAGGCTCCGCGGCGAGTACGCGCGCCTCAACTTCCCGGGCGCCATGGACGGGCCGGACCCGGACTGCCCCGAGCACCTGCGCCACCTCCGCGCCGCCGTCGACGCCAAGATCCAGGCCATCCGCGCCCGCATGGCGCGGAAGCGCGCGAGGGCGCGCAGGCAGCGCGAGGAGAGGGTCAGCGCCCGGTCCGGCTTGCAGGCCGCGTCGGAGGGCGCCGCGACGACGACAACGACGACGACCACCTCCGAGACGTCGGCGGCCACGCCGTGCGGGTCGCCCGACGGGGTGCTGTCCGTGAGCGCCGCCTCGGGCGTCGCCGACTGCCCGCTGGAACGGATGCCGTCGTTCGACCCGGAGCTCATCTGGGAGATGCTTAACTTC
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