| TFome pUT5375 | |
|---|---|
| Template | Genomic DNA |
| Genbank Insert ID | KJ727516 |
| Protein Name | ZmEREB96 |
| Species | Zea mays |
| TF Family | AP2/ERF-AP2 |
| Gene id | GRMZM2G044077 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT5375
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | SE0026_F |
| Temperature (ºC) | 62.3 |
| Sequence
|
caccATGTGGCCCATGGAGGAAG |
3' Primer
| Name | SE0026_R |
| Temperature (ºC) | |
| Sequence
|
GGCGACGGGCAGCGGG |
PCR Condition
mix per 25l reaction (5%DMSO): 5 l GC buffer 1.5l
Amino Acid Sequence
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MWPMEEEEVFVTVRRTEHVDVTSRAVVVAAGRAAVAGPRTVRVFCDDYDATDSSGDEAEEEEEKKAARRRVKRYVQEIHLERAAKEEEEATAAASLGAVLPGPKRKRKPDGEASEPRFRGVRRRPWGKYAAEIRDPWRRVRVWLGTFDTAEEAAKVYDSAAVQLRGRDATTNFQQQVDPERLSQPAASKSAATSTYDSGEESVTAVASPTSVLRSFPPSADAGGTRSKAAARNPETGDSTIVFGCPFSLASELPALYTEFDLLADLPLDFLADAPEVEALSLPPFSACTPEEEEECPSDAVRWRQVDELFQDITDLFQIDPLPVA
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTGGCCCATGGAGGAAGAGGAGGTGTTCGTGACGGTGAGGCGGACGGAGCACGTCGACGTCACCTCCCGGGCGGTGGTCGTGGCTGCGGGGCGCGCCGCCGTCGCGGGGCCCAGGACGGTGCGGGTCTTCTGCGACGACTACGACGCGACCGACTCGTCCGGCGACGAAGCGGAGGAGGAGGAGGAAAAGAAGGCCGCGCGCAGGAGGGTGAAGCGCTACGTGCAGGAGATCCACCTGGAGCGGGCGGCAAAGGAGGAGGAGGAGGCGACGGCCGCGGCGTCTCTGGGGGCGGTGCTCCCGGGGCCGAAGCGGAAGCGGAAGCCCGACGGTGAGGCCTCGGAGCCCCGGTTCCGCGGCGTGCGGCGGCGGCCGTGGGGCAAGTACGCGGCGGAGATCCGCGACCCGTGGCGCCGGGTGCGGGTGTGGCTCGGCACCTTCGACACCGCCGAGGAGGCCGCCAAGGTGTACGACTCGGCCGCCGTCCAGCTCCGCGGCCGCGACGCCACCACCAACTTCCAGCAGCAGGTGGACCCCGAGCGCCTCTCACAGCCGGCGGCGTCCAAGAGCGCCGCGACATCGACATACGACTCCGGCGAGGAGTCCGTGACGGCGGTGGCGTCGCCGACCTCCGTGCTCCGCTCGTTCCCGCCCTCCGCCGACGCCGGCGGCACACGCAGCAAGGCTGCCGCGAGGAACCCGGAGACCGGCGATTCGACCATCGTGTTCGGCTGCCCCTTCTCCTTGGCCAGCGAGCTCCCGGCGCTGTACACCGAGTTCGACCTGCTGGCCGACCTCCCGCTGGACTTCCTCGCCGACGCCCCGGAGGTGGAGGCGCTGTCCCTGCCGCCGTTCTCCGCCTGCACACCCGAGGAGGAGGAGGAGTGCCCGTCCGACGCGGTGAGGTGGCGGCAGGTGGACGAATTATTCCAAGACATCACCGACCTGTTCCAGATCGACCCGCTGCCCGTCGCC
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