TFome clone pUT5388

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B278_F
Temperature (ºC)	69.4
Sequence Copy to clipboard	caccATGGGAAGGGCACCCTGCTGCG

3' Primer

Name	B278_R
Temperature (ºC)
Sequence Copy to clipboard	CTTGAAAGCCGAGTCCAGGGACG

PCR Condition

PCR Reaction Mix: 8.75l ultrapure water 2.5l 10x t

Amino Acid Sequence

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MGRAPCCDKNKGLKKGPWTPEEDKLLVDYIQTNGHGSWRLLPKLAGLNRCGKSCRLRWTNYLRPDIKRGPFTAEEEKSIVQLHAIVGNKWSMIAAQLPGRTDNEIKNYWNTHLKKQLRRMGLDEPPPGPAAGCPAARHMAQWETARLEAEARLSLLAATAASSGTTATTTATTTTSAASSSSTTVAGAADNKASNPADIFLRLWSSDIGDSFRRKTDTTPAVPALPAPVVKRKDTAIKQEPRALLLGLRDDSSAASNDNETEVAEALEAKYQMFLDFAGEELGLFHGRHGGFSLFPPLDVLAEASLDSAFK

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGGAAGGGCACCCTGCTGCGACAAGAACAAGGGCCTCAAGAAGGGGCCCTGGACGCCGGAGGAGGACAAGCTCCTCGTCGACTACATCCAGACCAACGGCCATGGCAGCTGGCGCCTGCTCCCCAAGCTCGCAGGGCTGAACCGGTGCGGCAAGAGCTGCCGACTGCGGTGGACCAACTACCTCCGGCCGGACATCAAGCGCGGGCCCTTCACAGCCGAGGAGGAGAAGTCCATCGTCCAGCTCCACGCCATCGTCGGCAACAAGTGGTCCATGATCGCGGCGCAGCTCCCAGGCCGGACGGACAACGAGATCAAGAACTACTGGAACACGCACCTCAAGAAGCAGCTGCGCCGGATGGGCCTCGACGAGCCCCCGCCCGGCCCGGCCGCCGGCTGCCCCGCCGCGCGCCACATGGCGCAGTGGGAGACGGCCAGGCTCGAGGCCGAGGCGCGCCTCTCCCTCCTCGCCGCCACCGCCGCCTCCTCCGGCACCACCGCGACCACCACAGCCACCACCACCACGTCTGCAGCCTCCTCGTCCTCCACCACCGTCGCTGGCGCGGCCGACAACAAGGCCTCCAACCCCGCGGACATCTTCCTGCGCCTGTGGAGCTCGGACATCGGGGACTCCTTCCGCCGCAAGACGGACACGACGCCAGCGGTGCCCGCGCTTCCGGCGCCCGTCGTCAAGAGGAAGGACACGGCGATCAAGCAGGAGCCGCGGGCGCTGCTGCTCGGCCTCAGGGACGACTCCTCGGCGGCGTCCAACGACAACGAGACGGAGGTGGCGGAGGCGCTGGAGGCGAAGTACCAGATGTTCCTCGACTTCGCGGGCGAAGAGCTGGGATTGTTCCATGGCCGGCACGGCGGCTTCTCGCTGTTCCCGCCGCTCGACGTGCTCGCTGAGGCGTCCCTGGACTCGGCTTTCAAG