TFome clone pUT5392

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B309_F
Temperature (ºC)	69.5
Sequence Copy to clipboard	caccATGGAGAAGAGCCCGGGCGCC

3' Primer

Name	B309_R
Temperature (ºC)
Sequence Copy to clipboard	CTCACCTCCGCCACCATGCCACTCCA

PCR Condition

PCR Reaction Mix:5 l GC buffer (NEB) 0,5 l primer

Amino Acid Sequence

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MEKSPGAAPTSSPEEEVAADGRGHVEDATGQKEQATPVVLKKGPWTAAEDAMLMDHVRHHGEGNWNAVQRVTGLLRCGKSCRLRWTNHLRPNLKKGSFSPDEELLIAQLHAQLGNKWARMAAHLPGRTDNEIKNYWNTRTKRRQRAGLPVHPPPEVQLQLALARRCRYDDFSPLPSPQELSGVQALVGAAASAGYASSRPAPLDLARQLTQTTNQQTETVQFLSPPPFSAPSSPWARPFARNAQYFQLAHSSSPVSPTTPDLSLGYGARSAGADQSRLPPLSPSPCPRVVELPSNQYAQPTPPASAAATALGGGGAALPDHQNAASLEKMLRELHDVIKVDPPALVPASGGAALEWHGGGGE

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGAGAAGAGCCCGGGCGCCGCGCCGACATCATCGCCCGAGGAGGAGGTGGCGGCGGATGGCCGCGGGCACGTTGAGGACGCGACGGGGCAGAAGGAGCAGGCGACGCCGGTGGTGCTCAAGAAGGGGCCGTGGACGGCAGCGGAGGATGCCATGCTCATGGACCACGTCCGGCACCACGGCGAGGGGAACTGGAACGCAGTGCAGAGGGTGACCGGCCTGCTGCGCTGCGGCAAGAGCTGCCGCCTGCGCTGGACCAACCACCTCCGCCCCAACCTCAAGAAGGGCTCCTTCTCCCCCGACGAGGAGCTCCTCATCGCCCAGCTCCACGCGCAGCTCGGCAACAAGTGGGCGCGCATGGCCGCGCATCTGCCGGGGAGGACGGACAACGAGATCAAGAACTACTGGAACACGAGGACCAAGCGCCGCCAGCGCGCCGGCCTGCCTGTGCACCCGCCGCCAGAGGTGCAGCTCCAGCTCGCCCTCGCCAGGCGCTGCCGCTACGACGACTTCTCGCCGCTGCCGTCCCCGCAGGAGCTGTCCGGTGTCCAGGCGCTGGTGGGCGCCGCCGCCTCGGCCGGGTACGCCAGCTCCCGCCCGGCGCCCCTCGACCTGGCTCGGCAGCTCACCCAGACGACGAACCAGCAGACGGAGACTGTGCAGTTCCTCTCGCCGCCACCCTTCTCGGCGCCGTCGTCCCCGTGGGCGAGGCCGTTCGCCCGGAACGCGCAGTACTTCCAGCTCGCGCACTCGTCGTCGCCCGTGTCCCCGACGACGCCGGACCTGTCCCTCGGCTACGGCGCGCGCAGCGCCGGCGCCGACCAGAGCAGGCTCCCGCCGCTCTCGCCGTCCCCTTGCCCCAGAGTGGTGGAGCTCCCTTCAAACCAATACGCCCAACCGACGCCGCCGGCCTCGGCCGCCGCCACCGCACTTGGAGGTGGTGGCGCAGCGCTGCCGGATCATCAGAACGCGGCGAGCCTCGAGAAGATGCTGCGCGAGCTGCATGACGTCATCAAGGTCGACCCGCCGGCGCTGGTCCCGGCCAGCGGCGGCGCTGCCCTGGAGTGGCATGGTGGCGGAGGTGAG