TFome clone pUT5394

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B316_F
Temperature (ºC)	67.6
Sequence Copy to clipboard	caccATGACGCGGCGGTGCTCG

3' Primer

Name	B316_R
Temperature (ºC)
Sequence Copy to clipboard	AGCTTGAGCCCTCGTTTGAGGATTTGC

PCR Condition

PCR out optimization 5 l GC buffer (Thermo Fisher/

Amino Acid Sequence

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MTRRCSHCSHNGHNSRTCPNRGVKIFGVRLTDGSAIRKSASMGNLSLLSAGSTSGGASPADGPDLADGGGGYASDDFVQGSSSASRERKKGVPWTEEEHRRFLLGLQKLGKGDWRGISRNFVVSRTPTQVASHAQKYFIRQSNMSRRKRRSSLFDMVPDESMDLPPLPGSQEPETSVLNQPPLPPPVEEEEEVESMESDTSAVAESSAASALMPESLQPTYPMIVPAYFSPFLQFSVPFWPNQEDGGDLPQETHEIVKPVAVHSKNPINVDELVSMSKLSIGEPGQETVSTSLSLNLLVGQNRQSAFHANPQTRAQA

TFome clones do not contain stop codon at 3'end.

CDS

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ATGACGCGGCGGTGCTCGCACTGCAGCCACAACGGGCACAACTCGCGGACGTGCCCCAACCGCGGGGTCAAGATCTTCGGGGTGCGCCTCACCGATGGCTCGGCCATCCGCAAGAGCGCAAGCATGGGGAACCTCTCCCTCCTCTCCGCGGGGTCAACCAGCGGCGGCGCGTCGCCCGCCGACGGGCCCGACCTCGCCGACGGCGGCGGGGGCTACGCCTCCGACGACTTCGTCCAGGGGTCGTCCTCCGCCAGCCGCGAGCGTAAGAAAGGTGTTCCTTGGACTGAAGAAGAACACCGGAGGTTTTTGCTGGGATTACAAAAGCTTGGGAAAGGTGATTGGCGAGGGATTTCTCGTAATTTCGTGGTCTCAAGAACACCTACTCAAGTAGCAAGTCATGCTCAAAAATATTTTATACGTCAATCAAATATGAGCAGAAGGAAGAGAAGGTCTAGCCTTTTTGACATGGTGCCTGATGAGTCCATGGACCTTCCACCCCTTCCTGGAAGTCAAGAGCCAGAGACCTCAGTGTTAAATCAACCACCACTGCCTCCCCCTGTGGAGGAGGAGGAGGAGGTGGAATCGATGGAGTCAGATACTTCTGCTGTTGCGGAGAGTTCTGCAGCTTCAGCTCTTATGCCCGAGAGTTTACAGCCTACCTATCCGATGATTGTTCCAGCTTATTTCTCACCGTTCTTGCAATTCTCAGTTCCTTTCTGGCCAAATCAGGAAGATGGAGGTGATCTGCCTCAAGAAACGCACGAGATTGTCAAGCCTGTTGCAGTTCATTCCAAGAATCCAATTAATGTTGATGAACTTGTGAGCATGTCAAAGCTAAGCATAGGGGAGCCTGGTCAGGAAACGGTGTCTACTTCTCTGTCGTTAAATCTGCTGGTGGGTCAAAATAGGCAGTCGGCCTTCCATGCAAATCCTCAAACGAGGGCTCAAGCT