TFome clone pUT5403

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B351_F
Temperature (ºC)	60.5
Sequence Copy to clipboard	caccATGTCGTCGAGAGACTTATACTTCCA

3' Primer

Name	B351_R
Temperature (ºC)
Sequence Copy to clipboard	GGGCTGGATGTTACCGCCGC

PCR Condition

PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f

Amino Acid Sequence

Copy to clipboard

MSSRDLYFHDELASLFAQRPAPAGEAMMTHQHQQQQQAPASWFADYLHAGAPGMGMDYDLLCRALDLPLPGDDVVKREEELLLVDTGGDGGGFAAPTPTPSGGGTAPVTPNTTSSMSSSSSEAAGGAAGGGGGREEDSPQGRCKKEEGDGEESKALDKGEEDADKGKKGSAAAGKGKGKGEKRQRQPRFAFLTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCPVKKRVERSSQDPAVVITTYEGKHTHPIPVTLRGSTHILAAQLHHHHNHHLGAFPTPLPQTGAAPFGRAAGGGVIDMLGLLPPRNHGMSGGPMSAVAGAATTTAATITTTSTTPSYPPSLQMQHFMAQDFGLLQDIMLPSFGGGGGNIQP

TFome clones do not contain stop codon at 3'end.

CDS

Copy to clipboard

ATGTCGTCGAGAGACTTATACTTCCACGACGAGCTGGCGTCGCTGTTCGCGCAGCGGCCGGCGCCGGCCGGAGAGGCGATGATGACGCACCAGCACCAGCAGCAGCAACAGGCGCCGGCGTCGTGGTTCGCGGACTACCTGCACGCGGGCGCCCCAGGCATGGGGATGGACTACGACCTGCTGTGCCGCGCGCTGGACCTGCCGCTGCCGGGGGACGACGTCGTCAAGAGGGAGGAGGAGCTGCTGCTGGTGGACACAGGCGGGGACGGAGGAGGCTTCGCCGCGCCCACGCCCACGCCCAGCGGGGGCGGCACGGCGCCGGTCACGCCCAACACCACGTCGTCCATGTCCTCCTCGTCCAGCGAGGCGGCGGGCGGCGCCGCCGGAGGAGGAGGAGGAAGGGAGGAGGACTCGCCGCAGGGGAGGTGCAAGAAGGAGGAAGGGGATGGGGAGGAGAGCAAGGCGCTCGACAAGGGGGAGGAAGACGCTGACAAGGGCAAGAAAGGGTCAGCGGCGGCAGGCAAGGGCAAGGGCAAGGGCGAGAAGCGGCAGCGGCAGCCGCGGTTCGCGTTCTTGACCAAGAGCGAGGTCGACCACCTTGAGGACGGCTACCGGTGGCGCAAGTACGGCCAGAAGGCCGTCAAGAACAGCCCATACCCCAGGAGCTACTACCGGTGCACGACGCAGAAGTGCCCCGTGAAGAAGCGGGTGGAGCGGTCGTCCCAGGACCCGGCGGTGGTGATCACCACGTACGAGGGCAAGCACACGCACCCCATCCCGGTCACGCTCCGCGGCAGCACCCACATCCTCGCCGCGCAGCTGCACCACCACCACAACCACCACCTCGGCGCCTTCCCGACGCCGCTGCCGCAGACGGGCGCGGCGCCGTTCGGGCGGGCTGCAGGCGGAGGCGTCATCGACATGCTGGGGCTCCTGCCCCCGCGCAACCACGGCATGAGCGGCGGCCCGATGAGCGCCGTGGCTGGCGCCGCGACGACGACGGCCGCCACCATTACTACTACCTCCACCACACCGTCGTATCCCCCCTCGCTCCAGATGCAGCACTTCATGGCGCAGGACTTCGGGCTCCTGCAGGACATCATGCTGCCGTCCTTCGGCGGCGGCGGCGGTAACATCCAGCCC