| TFome pUT5423 | |
|---|---|
| Template | BT035373.1 |
| Genbank Insert ID | KJ727563 |
| Protein Name | ZmMADS12 |
| Species | Zea mays |
| TF Family | MADS |
| Gene id | GRMZM2G160565 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5423
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B577_F |
| Temperature (ºC) | 73.5 |
| Sequence
|
caccATGGGGAGGGGACGAGTT |
3' Primer
| Name | B577_R |
| Temperature (ºC) | |
| Sequence
|
AAGAACCCATCCCAGCATGAAGTT |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f
Amino Acid Sequence
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MGRGRVELKRIENKINRQVTFSKRRNGLLKKAYELSVLCDAEVALIIFSSRGKLYEFGSAGITKTLERYQHCCYNAQDSNGALSETQSWYQEMSKLRAKFEALQRTQRHLLGEELGPLSVKELQQLEKQLECALSQARQRKTQLMMEQVEELRRKERHLGEMNRQLKHKLEAEGCSNYRTLQHAAWPAPGSTMVEHDGATYHVHPTTAQSVAMDCEPTLQIGYPPHHQFLPSEAANNIPRSPPGGENNFMLGWVL
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGGAGGGGACGAGTTGAGCTCAAGCGGATCGAGAACAAGATCAACCGCCAGGTCACCTTCTCCAAGCGCCGCAACGGCCTGCTCAAGAAGGCCTACGAGCTCTCCGTGCTCTGCGACGCCGAGGTCGCGCTCATCATCTTCTCCAGCCGCGGCAAGCTCTACGAGTTCGGCAGCGCCGGCATAACAAAAACTTTAGAAAGGTACCAACATTGCTGCTACAATGCTCAAGATTCCAATGGCGCACTCTCTGAAACTCAGAGCTGGTACCAGGAAATGTCAAAACTGAGGGCAAAATTCGAGGCCTTGCAGCGCACTCAGAGGCACTTGCTTGGGGAGGAACTTGGCCCACTGAGTGTGAAGGAGTTGCAGCAGCTAGAGAAACAGCTCGAATGTGCTTTGTCACAGGCAAGACAGAGAAAGACACAACTTATGATGGAGCAAGTGGAAGAGCTCCGCAGAAAGGAGCGACACCTGGGAGAAATGAACAGGCAACTCAAACACAAGCTTGAAGCTGAAGGTTGTAGCAACTACAGAACCCTGCAGCATGCAGCCTGGCCAGCTCCCGGCAGCACCATGGTGGAGCATGACGGCGCCACCTATCATGTGCATCCAACAACTGCTCAATCGGTTGCAATGGACTGTGAACCCACTCTGCAAATCGGGTACCCTCCTCATCACCAGTTTCTGCCTTCCGAGGCAGCCAATAATATCCCAAGGAGCCCCCCTGGAGGCGAGAACAACTTCATGCTGGGATGGGTTCTT
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