| TFome pUT5428 | |
|---|---|
| Template | BT087336 |
| Genbank Insert ID | KJ727568 |
| Protein Name | ZmWRKY102 |
| Species | Zea mays |
| TF Family | WRKY |
| Gene id | GRMZM2G070211 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.A405G p. (=) Not present in template FLcDNA. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5428
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B741_F |
| Temperature (ºC) | 63.2 |
| Sequence
|
caccATGGAGGAAGTGGAGGAAGCG |
3' Primer
| Name | B741_R |
| Temperature (ºC) | |
| Sequence
|
CACCTGTGCTGACTGAGTTGTTGGC |
PCR Condition
40 sec 98°C 30x : 15 sec 98°C 30 sec 58&de
Amino Acid Sequence
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MEEVEEANREAVESCHRVLALLSQPHDPAQARSIALGTDEACARFRKVVSLLSNGGAGLGEAGPSCGSASASRPRAKLVSRRQNPGFLTQKGFLDSNTPVVVLNSAHPSTTSAQVYPRTGALVDAQSVHPLGVGGPPKLVQPLSAHFQFGSVPARYQFPNQQQQKLHAEMFKRSNSGVNLKFESASGTAGTMSSARSFLSSLSMDGSVASLDAKSSSFHLIGGPAMSDPLNAQQPPRRRCTGRGEDGTGKCAVTGRCHCSKRSRKLRVKRSIKVPAISNKIADIPPDEYSWRKYGQKPIKGSPHPRGYYKCSSVRGCPARKHVERCVDDSAMLIVTYEGEHNHTRMPTTQSAQV
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAGGAAGTGGAGGAAGCGAACAGGGAAGCCGTGGAGAGCTGCCACAGGGTGCTCGCCTTGCTCTCCCAGCCGCATGACCCCGCGCAGGCCAGGAGCATAGCTCTGGGCACGGACGAAGCATGTGCCAGGTTCAGGAAGGTGGTCTCTCTACTGAGCAATGGAGGAGCGGGGCTAGGAGAAGCCGGGCCGTCATGCGGAAGCGCAAGCGCAAGCCGTCCTAGAGCTAAGCTTGTTAGCAGAAGACAGAATCCAGGGTTCTTGACCCAGAAAGGCTTCCTGGACAGCAACACCCCGGTCGTGGTGTTGAACAGCGCCCATCCTTCTACTACCTCCGCGCAGGTGTACCCTAGAACTGGAGCTCTCGTCGATGCACAGAGCGTGCACCCCCTCGGAGTCGGAGGGCCTCCCAAGCTGGTCCAGCCCTTGTCCGCGCACTTTCAGTTCGGCAGTGTGCCGGCTCGGTATCAGTTCCCGAATCAGCAGCAGCAGAAGTTGCACGCCGAGATGTTCAAGAGAAGCAACAGCGGGGTTAACCTGAAGTTCGAGAGCGCCAGTGGCACTGCTGGGACGATGTCGTCGGCGAGGTCCTTCTTGTCGTCTCTGAGCATGGACGGTAGCGTGGCTAGCCTGGACGCCAAGTCATCGTCGTTCCATTTGATCGGTGGACCTGCGATGAGCGATCCGCTGAACGCGCAGCAGCCCCCGAGGAGGCGGTGCACGGGCCGTGGGGAGGATGGGACCGGCAAGTGCGCTGTTACAGGGAGGTGCCACTGTTCGAAGAGAAGTAGGAAGTTGCGGGTGAAGAGGTCGATTAAGGTTCCTGCCATTAGTAACAAGATTGCCGATATACCTCCGGATGAATACTCGTGGAGGAAGTATGGGCAGAAGCCAATTAAGGGTTCCCCTCATCCTAGGGGTTACTACAAATGCAGTAGCGTGAGGGGCTGCCCAGCCAGGAAGCATGTGGAACGGTGCGTAGATGATTCGGCGATGCTCATCGTGACATATGAGGGCGAGCACAACCACACCAGAATGCCAACAACTCAGTCAGCACAGGTG
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