TFome clone pUT5429

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B742_F
Temperature (ºC)	61.9
Sequence Copy to clipboard	caccATGGATCAGAGCTTCGGGAATCT

3' Primer

Name	B742_R
Temperature (ºC)
Sequence Copy to clipboard	TGAACCGAGGCGATACATGCCG

PCR Condition

40 sec 98°C 30x : 15 sec 98°C 30 sec 58&de

Amino Acid Sequence

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MDQSFGNLGAGAGSSSGGSNSKAAAAAVSSSSFLQLPLSTASPAYYGAPLALLHHAAAAPSSSQQHQQQQHHHHYARHGAEMSAAEAEAIKAKIVAHPQYSALLAAYLDCQKVGAPPDVLERLTAMAAKLDARPPGRHEPRDPELDQFMEAYCNMLVKYREELTRPIDEAMEFLKRVEAQLDCISGGGGSSSARLSLADGKSEGVGSSEDDMDPNGRENDPPEIDPRAEDKELKYQLLKKYSGYLSSLRQEFSKKKKKGKLPKEARQKLLHWWELHYKWPYPSETEKIALAESTGLDQKQINNWFINQRKRHWKPSEDMPFVMMEGFHPQNAAALYMDGPFMADGMYRLGS

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGATCAGAGCTTCGGGAATCTCGGGGCCGGAGCTGGGAGCAGCAGCGGGGGCTCCAACTCCAAGGCGGCGGCAGCCGCCGTGTCCTCCTCGTCTTTCCTGCAGCTGCCGCTGTCGACGGCGTCCCCCGCGTACTACGGCGCGCCGCTCGCGCTCCTGCACCACGCCGCCGCGGCCCCGTCGTCGTCGCAGCAGCACCAGCAGCAGCAGCACCACCACCACTACGCCAGGCACGGCGCCGAGATGTCGGCAGCGGAGGCCGAGGCCATCAAGGCCAAGATCGTGGCGCACCCGCAGTACTCGGCGCTCCTCGCCGCCTACCTCGACTGCCAGAAAGTGGGCGCGCCGCCGGACGTGCTGGAGAGGCTGACGGCCATGGCGGCAAAGCTGGACGCGCGCCCGCCGGGCCGCCACGAGCCGCGCGACCCGGAGCTGGACCAGTTCATGGAGGCGTACTGTAACATGCTCGTCAAGTACCGGGAGGAGCTCACCCGGCCGATCGACGAGGCCATGGAGTTCCTCAAGCGGGTGGAGGCGCAGCTCGACTGCATCTCCGGCGGCGGCGGCTCCTCCTCAGCGCGCCTCTCGCTCGCCGATGGTAAATCAGAAGGTGTTGGCTCTTCTGAAGATGACATGGACCCGAATGGTCGCGAGAACGATCCGCCGGAGATTGACCCACGAGCGGAGGACAAGGAGCTCAAGTACCAGCTGCTGAAGAAGTACAGTGGCTACCTGAGCAGCCTCAGGCAGGAGTTTTCCAAGAAGAAGAAGAAAGGGAAGCTCCCAAAGGAGGCCAGGCAGAAACTCCTCCACTGGTGGGAGCTGCACTACAAGTGGCCGTACCCTTCAGAGACGGAGAAGATTGCGCTCGCAGAATCAACGGGCCTAGACCAGAAGCAGATCAACAACTGGTTCATCAACCAGAGGAAACGGCACTGGAAGCCATCGGAGGACATGCCTTTCGTGATGATGGAAGGCTTCCACCCGCAGAACGCCGCTGCTCTGTACATGGACGGGCCGTTCATGGCCGACGGCATGTATCGCCTCGGTTCA