| PCR Condition:
|
40 sec 98°C 30x : 15 sec 98°C 30 sec 58&de |
| Nucleotide Sequence:
|
ATGTCGTCGGGCGAGGACGGGTACTGCAGCTCGGATTCGCCGCGGGCGGAATCCCCCGACCAGCCGCAGGCAGCTGCGGCGGCGGACGCGGACGCGGACGCGGAGTCGCCTCGCGCCGGAGCCGGGCCGAACAAGCGCGACCTCCACGCCTCGTCGCCGGCAGCTAAGCGCAGCCGGAGATCGGTGGAGAAGCGGGTTGTGTCGGTGCCGCTGGCCGAGTGCGGGGAGGGGCCGCCGCCGTCTGACTCGTGGGCGTGGCGCAAGTATGGGCAGAAGCCCATCAAGGGCTCCCCCTACCCACGTGGGTACTACCGCTGCAGCAGCTCCAAGGGGTGCCCCGCGAGGAAGCAGGTGGAGCGCAGCCGCGCCGACCCTGCCGTGCTGCTCGTCACCTACACCTTCGACCACAACCACGAGGCGCCGCGGCCCAAGAGCAGCTGCTGCCACCAGCAAGGCAGGCCGCCGGCGCCGAAGCCTGATGAGCCCGTGGCCGAGCAGGAGGATGAGCTGGGTCCGGAGCATGAGCCGGAAGACGAGCAGGAGCCGGAGCAGAAGGTCGTCCCAGGTCTGGCCGGGCCGGAAACGGCGCCGGCAACCGCAACGTTGGCGCCGGTGGCAGAGGAGGACGAAAGCTTCGACTTGGGGTGGTTCGACCAGTACCCGACGTGGCACCGTTCGGCGCTGTACGCGCCGCTGCTGCCGCCGGAGGAGTGGGAGCGGGAGCTGCAGGGGGAAGACGCCCTGTTCGCGGGCCTCGGCGAGCTGCCCGAGTGCGCCGTCGTGTTCGGCCGCCGCCGCGAGCTCAGCCTGGCCGCTACCGCGCCGTGCTCC |
|
|
TFome clones do not contain stop codon at 3'end.
|
| translation:
|
MSSGEDGYCSSDSPRAESPDQPQAAAAADADADAESPRAGAGPNKRDLHASSPAAKRSRRSVEKRVVSVPLAECGEGPPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRADPAVLLVTYTFDHNHEAPRPKSSCCHQQGRPPAPKPDEPVAEQEDELGPEHEPEDEQEPEQKVVPGLAGPETAPATATLAPVAEEDESFDLGWFDQYPTWHRSALYAPLLPPEEWERELQGEDALFAGLGELPECAVVFGRRRELSLAATAPCS
|
