| TFome pUT5430 | |
|---|---|
| Template | BT087259 |
| Genbank Insert ID | KJ727570 |
| Protein Name | ZmWRKY111 |
| Species | Zea mays |
| TF Family | WRKY |
| Gene id | GRMZM2G013391 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT5430
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B749_F |
| Temperature (ºC) | 63.9 |
| Sequence
|
caccATGTCGTCGGGCGAGGA |
3' Primer
| Name | B749_R |
| Temperature (ºC) | |
| Sequence
|
GGAGCACGGCGCGGTAGC |
PCR Condition
40 sec 98°C 30x : 15 sec 98°C 30 sec 58&de
Amino Acid Sequence
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MSSGEDGYCSSDSPRAESPDQPQAAAAADADADAESPRAGAGPNKRDLHASSPAAKRSRRSVEKRVVSVPLAECGEGPPPSDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRADPAVLLVTYTFDHNHEAPRPKSSCCHQQGRPPAPKPDEPVAEQEDELGPEHEPEDEQEPEQKVVPGLAGPETAPATATLAPVAEEDESFDLGWFDQYPTWHRSALYAPLLPPEEWERELQGEDALFAGLGELPECAVVFGRRRELSLAATAPCS
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTCGTCGGGCGAGGACGGGTACTGCAGCTCGGATTCGCCGCGGGCGGAATCCCCCGACCAGCCGCAGGCAGCTGCGGCGGCGGACGCGGACGCGGACGCGGAGTCGCCTCGCGCCGGAGCCGGGCCGAACAAGCGCGACCTCCACGCCTCGTCGCCGGCAGCTAAGCGCAGCCGGAGATCGGTGGAGAAGCGGGTTGTGTCGGTGCCGCTGGCCGAGTGCGGGGAGGGGCCGCCGCCGTCTGACTCGTGGGCGTGGCGCAAGTATGGGCAGAAGCCCATCAAGGGCTCCCCCTACCCACGTGGGTACTACCGCTGCAGCAGCTCCAAGGGGTGCCCCGCGAGGAAGCAGGTGGAGCGCAGCCGCGCCGACCCTGCCGTGCTGCTCGTCACCTACACCTTCGACCACAACCACGAGGCGCCGCGGCCCAAGAGCAGCTGCTGCCACCAGCAAGGCAGGCCGCCGGCGCCGAAGCCTGATGAGCCCGTGGCCGAGCAGGAGGATGAGCTGGGTCCGGAGCATGAGCCGGAAGACGAGCAGGAGCCGGAGCAGAAGGTCGTCCCAGGTCTGGCCGGGCCGGAAACGGCGCCGGCAACCGCAACGTTGGCGCCGGTGGCAGAGGAGGACGAAAGCTTCGACTTGGGGTGGTTCGACCAGTACCCGACGTGGCACCGTTCGGCGCTGTACGCGCCGCTGCTGCCGCCGGAGGAGTGGGAGCGGGAGCTGCAGGGGGAAGACGCCCTGTTCGCGGGCCTCGGCGAGCTGCCCGAGTGCGCCGTCGTGTTCGGCCGCCGCCGCGAGCTCAGCCTGGCCGCTACCGCGCCGTGCTCC
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