TFome clone pUT5433

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B763_F
Temperature (ºC)	63.3
Sequence Copy to clipboard	caccATGGAGGAAGAGGAGGGATCGT

3' Primer

Name	B763_R
Temperature (ºC)
Sequence Copy to clipboard	GAAAATGTTGGAGAGAATGGCCATG

PCR Condition

40 sec 98°C 30x : 15 sec 98°C 30 sec 58&de

Amino Acid Sequence

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MEEEEGSSTSSVAVPVPPPPNKYDSWTTDPEELMMMMVDEAAVQQEGPRRRRDSMLNKLISTVYSGPTISDIESALSFTGGGDVDARNYNNSSAGPGALSSPEKVLMSKMENKYTLKIKTCGNGSSLAEDGYKWRKYGQKSIKNSPNPRSYYRCTNPRCNAKKQVERSTDEPDTLVVTYEGLHLHYTYSHFLHHQQPPPQPKKPKLGAGPPQQPIVVMDDDLLHGPAQQDIITTGPLGAAVMAPAPAPPPAAASFCYDLDDDVPAFFDDHDHHHHQQQHITNGGLLEDMVPLLVRRPCTTTTTTSAGSTSPDLSASSSVSWNPTSPYIDMAILSNIF

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGAGGAAGAGGAGGGATCGTCGACATCATCAGTCGCTGTGCCTGTGCCGCCGCCGCCTAATAAGTACGACTCCTGGACGACAGATCCGGAGGAGCTGATGATGATGATGGTCGACGAGGCGGCGGTGCAGCAGGAAGGGCCCCGTCGCCGGCGGGACTCCATGCTCAACAAGCTCATCTCCACGGTCTACTCCGGACCCACCATCAGCGACATAGAGAGCGCGCTCTCTTTCACCGGCGGCGGCGACGTGGATGCTCGCAACTACAACAACTCCTCCGCCGGCCCAGGGGCTTTATCGTCCCCGGAGAAGGTGCTGATGAGCAAGATGGAGAACAAGTACACGCTCAAGATCAAGACCTGTGGGAACGGCAGCAGCCTTGCGGAGGATGGATACAAATGGAGGAAATACGGCCAGAAATCCATCAAGAACAGCCCCAACCCAAGGAGCTACTACCGGTGCACGAACCCTCGGTGCAACGCCAAGAAGCAGGTGGAGCGGTCGACGGACGAGCCGGACACGCTCGTCGTCACCTACGAGGGCCTCCACCTGCACTACACCTACTCGCACTTCCTCCACCACCAGCAACCGCCGCCGCAGCCCAAAAAGCCCAAGCTCGGCGCCGGCCCACCACAACAGCCCATCGTCGTCATGGACGACGACCTTCTCCACGGCCCGGCCCAACAAGACATCATCACCACCGGCCCACTGGGAGCTGCTGTCATGGCTCCAGCACCAGCACCACCACCAGCAGCAGCATCTTTCTGCTACGACCTTGACGACGATGTACCTGCTTTTTTCGACGACCACGACCACCACCACCACCAGCAGCAGCACATCACCAACGGCGGGCTGCTGGAAGACATGGTGCCGCTGCTGGTCCGGCGGCCCTGCACCACCACCACCACCACCTCCGCCGGTAGCACGTCGCCGGACCTTTCCGCGTCGTCCTCTGTCTCATGGAACCCCACCTCGCCTTACATCGACATGGCCATTCTCTCCAACATTTTC