| TFome pUT5459 | |
|---|---|
| Template | BT033992.1 |
| Genbank Insert ID | KJ727599 |
| Protein Name | ZmbZIP108 |
| Species | Zea mays |
| TF Family | bZIP |
| Gene id | GRMZM2G033230 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT5459
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C249_F |
| Temperature (ºC) | 62.9 |
| Sequence
|
caccATGGACGATGGAGCTGACCTC |
3' Primer
| Name | C249_R |
| Temperature (ºC) | |
| Sequence
|
ATTTATCTTTAAAGCAAAGCCCAAAGAAGC |
PCR Condition
PCR Reaction Mix:5 l GC buffer 1 l primer forward
Amino Acid Sequence
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MDDGADLPCQLLFSHPEMPHSFDEFFSSATTTTTCTHTHSCDPPGPSAAMHTHTCLHTHTQVLGSGGEDDGSAKPRRPLGNREAVRKYREKKKARAAFLEGEVKKLRAANQQLVRRLQDHAALEAEVARLRGLLLDVRGRIDAEVGVSPFQRPCSAGSVECGADPALRFDGSSEVVGGGGWEKSSSPQVADCRIDEGGGGMSRELDVLEVSGPLPASLGFALKIN
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACGATGGAGCTGACCTCCCCTGCCAGTTGCTCTTCTCCCACCCGGAGATGCCTCACAGCTTCGACGAGTTCTTCAGCAGCGCCACGACCACGACCACCTGCACCCACACGCACAGCTGCGACCCTCCGGGCCCCTCGGCCGCCATGCATACCCACACGTGCCTGCACACGCACACCCAGGTCCTGGGCAGCGGCGGGGAGGACGACGGCTCAGCGAAGCCGCGGAGGCCCCTGGGAAACAGGGAAGCCGTGCGCAAGTACCGCGAGAAGAAGAAGGCGCGCGCGGCCTTCCTGGAAGGGGAGGTCAAGAAGCTGCGCGCCGCCAACCAGCAGCTCGTGCGGCGGCTGCAAGACCACGCGGCTCTGGAGGCGGAGGTGGCGAGGCTCAGGGGCCTCCTGCTCGACGTCCGGGGCAGGATCGACGCCGAGGTCGGCGTGTCCCCGTTTCAGAGGCCATGCAGCGCGGGCTCTGTGGAGTGCGGCGCTGATCCAGCTCTGCGCTTCGATGGCAGCTCGGAGGTAGTAGGAGGTGGTGGCTGGGAGAAGAGCTCAAGCCCTCAAGTTGCGGACTGCAGGATCGATGAAGGGGGAGGAGGGATGTCACGGGAACTTGATGTTCTAGAAGTTTCGGGGCCTCTGCCAGCTTCTTTGGGCTTTGCTTTAAAGATAAAT
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