| TFome pUT5524 | |
|---|---|
| Template | Full-length cDNA |
| Genbank Insert ID | KJ727613 |
| Protein Name | ZmWRKY10 |
| Species | Zea mays |
| TF Family | WRKY |
| Gene id | GRMZM2G083717 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5524
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B798_F |
| Temperature (ºC) | 67.8 |
| Sequence
|
caccATGGACAGCATGGAGGACG |
3' Primer
| Name | B798_R |
| Temperature (ºC) | |
| Sequence
|
GAACTTGTGCCACTCATAAGTCACCC |
PCR Condition
5 l GC buffer 1l MgCl2 50mM 1.25 l DMSO 100% 1 l d
Amino Acid Sequence
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MDSMEDAVSGTAAQGDLAEVVARAGAMAAAASHHHHRRPPSPAAADHGHIVSAARATARSQIMLVPAAACYDDETRPAAACGDAVMFDAPSSSMAAAVGPYHHLSSSSAALAPAHGGYWLPPQQISQEHARDGGLDEPPVVTRISPVTTPPPPSHHHQIMNRKNEVKKVVCIPALPPPASSRPGGGEVIPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCMARKQVERSRSDPNMLVITYTAEHNHPWPMQRNVLAGYSRPHTATHMSSNCKKKKNSCSVEPTSSWPTTTTSSPSSKNGNYECQQMTNMTMEENAAAGYVAYAIDDEECAAMHQPINCGSVQPSADEVFAELEELEPTTSPVNANIFSRGVTYEWHKF
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACAGCATGGAGGACGCCGTCTCCGGCACTGCAGCCCAGGGCGACCTCGCCGAGGTTGTGGCCCGCGCAGGTGCAATGGCCGCCGCGGCGAGTCACCACCACCACCGGCGGCCACCCTCTCCCGCAGCAGCAGATCACGGCCACATCGTGTCTGCTGCAAGGGCCACCGCCAGATCACAGATCATGCTCGTCCCTGCTGCTGCTTGCTACGACGACGAGACACGGCCTGCTGCTGCTTGCGGCGACGCGGTGATGTTCGACGCGCCGTCGTCGTCGATGGCCGCCGCCGTCGGTCCATACCACCACCTGTCGTCTTCGTCTGCGGCGCTGGCACCGGCGCACGGCGGGTACTGGCTGCCGCCGCAACAGATCTCCCAGGAGCACGCCCGCGACGGCGGCCTCGACGAGCCGCCCGTGGTGACGAGGATCTCGCCAGTGACGACTCCACCGCCGCCTTCTCATCATCATCAAATCATGAACAGAAAGAATGAGGTGAAGAAGGTGGTGTGCATCCCGGCGCTACCACCTCCGGCGAGCAGCCGCCCAGGAGGAGGAGAGGTGATTCCGTCTGATCTTTGGGCGTGGAGGAAATACGGCCAGAAGCCTATCAAGGGTTCGCCTTACCCAAGAGGTTACTACAGATGCAGCAGCTCAAAGGGATGTATGGCAAGGAAGCAAGTAGAGCGCAGTCGCAGCGACCCCAACATGCTCGTGATCACCTACACGGCAGAACACAACCACCCTTGGCCAATGCAACGCAATGTGCTCGCTGGCTACTCCCGGCCTCACACTGCTACTCACATGTCGTCCAACTGCAAGAAGAAGAAGAACAGCTGCAGCGTTGAACCGACGAGTTCATGGCCAACAACAACAACATCATCACCATCATCAAAGAACGGCAACTACGAGTGTCAGCAAATGACAAACATGACGATGGAAGAAAATGCAGCAGCAGGGTATGTTGCCTACGCCATCGATGACGAGGAGTGTGCCGCGATGCATCAGCCGATCAATTGCGGTAGCGTGCAACCTTCAGCAGATGAGGTTTTCGCAGAGCTAGAGGAATTGGAGCCTACGACTAGTCCTGTGAATGCAAACATCTTCTCTAGGGGGGTGACTTATGAGTGGCACAAGTTC
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