| TFome pUT5525 | |
|---|---|
| Template | BT067525.1 |
| Genbank Insert ID | KJ727614 |
| Protein Name | ZmMYB23 |
| Species | Zea mays |
| TF Family | MYB |
| Gene id | GRMZM2G150841 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.G649A p.G217R Not present in template FLcDNA. Not present in functional domain. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5525
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C147_F |
| Temperature (ºC) | 65.1 |
| Sequence
|
caccATGGCGAGGCGGACGAG |
3' Primer
| Name | C147_R |
| Temperature (ºC) | |
| Sequence
|
ATTCAAGCACCAACTGCTGC |
PCR Condition
PCR Reaction Mix:5 l GC buffer (NEB) 1 l primer fo
Amino Acid Sequence
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MARRTSGPKKKLRRGLWSPEEDDKLVNHIAKYGHGCWSSVPKLAGLERCGKSCRLRWINYLRPDLKRGAFSQEEEDLIIHLHSMMGNKWSQIASQLPGRTDNEVKNFWNSYVKKKLRQRGIDPATHKPLPLAAEGISSRTAVFSDAELILSSSAAAAAAGRHMPPPPVAASAAESYVYSYSHSSGGASDGSLQSLSGYNSNSKQTAADHLGAAAAARYLQQEQDPAAAAAAADALHCGPSGAPAVVLPSVSSSSTLNSMAGLSPAATTTDEQCTNNCSSCSFHSGLEPWLELGSTSGYYGAALDELKWSDYVFDGGYGGQPGQGIYGDSKDAVQFVDANALSSSSWCLN
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCGAGGCGGACGAGCGGCCCCAAGAAGAAGCTGAGGAGGGGCCTCTGGTCGCCGGAGGAGGACGATAAGCTCGTCAACCACATTGCCAAGTATGGCCACGGCTGCTGGAGTTCCGTCCCCAAGCTAGCAGGTCTAGAGAGGTGTGGGAAGAGCTGCAGGCTGAGGTGGATCAACTACCTCAGGCCGGACCTCAAGAGGGGAGCGTTCTCGCAGGAAGAGGAGGACCTCATCATCCATCTCCATTCCATGATGGGCAACAAGTGGTCTCAGATTGCTTCGCAGCTGCCGGGGCGGACGGACAACGAGGTGAAGAACTTCTGGAACTCGTACGTCAAGAAGAAGCTCCGGCAGCGCGGGATCGACCCGGCCACGCACAAGCCGCTCCCGCTGGCCGCCGAGGGCATCAGCAGCCGCACGGCGGTGTTCAGCGACGCCGAGCTCATCCTGTCGTCGTCGGCGGCGGCGGCGGCGGCGGGGCGGCACATGCCGCCACCGCCAGTGGCGGCATCGGCGGCGGAGAGCTACGTGTACAGCTACAGCCACAGCAGTGGCGGCGCGAGCGACGGCTCTCTGCAGTCGCTGTCGGGGTACAACAGCAACAGCAAGCAAACAGCTGCGGATCACTTGGGTGCCGCAGCCGCAGCGAGGTACCTGCAGCAGGAGCAGGACCCAGCAGCAGCAGCAGCAGCAGCCGACGCGCTGCACTGCGGACCCTCCGGTGCCCCGGCGGTCGTTCTCCCGTCGGTGTCCAGCTCCAGCACGCTGAACTCGATGGCTGGCCTAAGCCCCGCGGCCACCACCACCGACGAACAGTGCACCAACAACTGTAGCAGCTGCAGCTTCCACAGCGGGTTGGAGCCGTGGCTGGAGCTGGGCAGCACGAGCGGCTACTACGGCGCGGCGCTGGACGAGCTCAAGTGGTCCGACTACGTGTTCGACGGCGGCTACGGTGGCCAGCCGGGACAGGGCATCTACGGCGACAGCAAGGACGCCGTGCAGTTCGTGGACGCCAACGCGCTCAGCAGCAGCAGTTGGTGCTTGAAT
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