TFome clone pUT5565

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B303_F
Temperature (ºC)	68.4
Sequence Copy to clipboard	caccATGGCCACGACACAGA

3' Primer

Name	B303_R
Temperature (ºC)
Sequence Copy to clipboard	TTGCATCTTCCAAATTTCCTCCA

PCR Condition

PCR Reaction Mix: Volumes per reaction (25l): 5 l

Amino Acid Sequence

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MATTQSCQSRSSACSKAAACFPAAVAVDEEHGHHSHQLKGGAQEEAENDNNKPELRRGPWTVDEDLTLVNYIADNGEGRWNNLARAAGLKRTGKSCRLRWLNYLRPDVKRGNFSADEQLLILDLHTRWGNRWSKIAQHLPGRTDNEIKNYWRTRVQKHAKQLNCDANSKRFKDAMRYLWMPHLADDVDTIAAANDDDEDHHHHLRLLVLHHHQAQHLQQAAAAAGGAANDLAAGAYDVRQLHALPSSGMAATSSSDSLASESYDDGGLLFANLRAGEMLMDGGDWAAQQEADQGLWPPPPPPPSDLDQSVVQAAGAGAGQFQDMELSGWVQGFSESITDNFWALEEIWKMQ

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGCCACGACACAGAGCTGTCAGAGCAGGAGCAGCGCCTGCAGCAAGGCTGCTGCTTGCTTCCCGGCCGCCGTAGCGGTCGACGAGGAGCACGGCCACCACAGCCACCAGCTGAAGGGAGGAGCGCAGGAGGAGGCTGAGAACGACAATAATAAGCCGGAGCTCCGGCGTGGCCCCTGGACGGTAGACGAGGACCTCACCCTCGTCAACTACATCGCCGACAACGGCGAGGGTCGCTGGAACAACCTCGCCCGCGCCGCCGGGCTGAAGCGGACGGGCAAGAGCTGCCGGCTGCGGTGGCTCAACTACCTCCGGCCCGACGTGAAGCGTGGCAACTTCAGCGCCGACGAGCAGCTGCTCATCCTCGACCTCCACACCCGCTGGGGCAACCGATGGTCGAAGATAGCGCAGCACCTGCCGGGAAGGACGGACAACGAGATCAAGAACTACTGGAGGACCCGGGTGCAGAAGCACGCCAAGCAGCTCAACTGCGACGCCAACAGCAAGCGCTTCAAGGACGCCATGCGCTACCTCTGGATGCCGCACCTCGCCGACGACGTCGATACCATCGCTGCGGCCAACGACGACGACGAAGACCACCACCACCACCTACGCCTCCTCGTCCTGCACCACCACCAGGCCCAGCACCTGCAGCAAGCTGCTGCCGCGGCCGGCGGCGCTGCCAACGACCTTGCTGCGGGCGCCTACGACGTCCGCCAGCTGCACGCGCTGCCGTCGTCGGGCATGGCGGCGACGTCGTCGTCCGACTCGCTCGCGTCGGAGTCGTACGATGACGGAGGCCTGCTTTTCGCGAACTTGCGCGCCGGCGAGATGCTGATGGACGGCGGAGATTGGGCGGCGCAGCAGGAGGCCGACCAAGGGCTGTGGCCGCCGCCGCCGCCGCCGCCGTCTGATCTTGATCAGTCGGTGGTGCAGGCTGCTGGTGCCGGCGCTGGCCAGTTTCAGGACATGGAGCTCAGTGGTTGGGTGCAAGGCTTCTCCGAGAGCATTACAGATAACTTTTGGGCCTTGGAGGAAATTTGGAAGATGCAA