| TFome pUT5566 | |
|---|---|
| Template | BT056102.1 |
| Genbank Insert ID | KJ727639 |
| Protein Name | ZmEREB27 |
| Species | Zea mays |
| TF Family | AP2/ERF-ERF |
| Gene id | GRMZM2G084264 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.G168T p.(=) Not present in template FlcDNA. c.T226C p.F76L Present in template FlcDNA Not present in functional domains. |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT5566
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B368_F |
| Temperature (ºC) | 71.5 |
| Sequence
|
caccATGGTGAAGACGGCGGCGAG |
3' Primer
| Name | B368_R |
| Temperature (ºC) | |
| Sequence
|
GCAGTCCAGTGTAGCGGAGAAGCTCCA |
PCR Condition
Volumes per reaction (25l): 5 l GC buffer (Thermo
Amino Acid Sequence
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MVKTAASSSSDDAAAAKRRTYKGVRMRSWGSWVSEVRAPGQKTRIWLGSHATAEAAARAHDAALLCLRGSAADLNLPLRLPFDLPPAATMSPKAIQRVAAAAASASSSSSCGGGGGLRAPPPFAPRAENNGTSACSDGDGDATPASSTTSSPTSHADDVSSPDSTVSSAGDVDYYTSLADIDAFFQSPKCVVDYALMDPCSAFFAPDSTAADDDGGCCWAWEEDGGGIALWSFSATLDC
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGTGAAGACGGCGGCGAGCAGCAGCAGCGACGACGCCGCCGCCGCCAAGAGGAGGACGTACAAGGGCGTGCGGATGCGGAGCTGGGGCTCGTGGGTGTCGGAGGTGCGAGCGCCCGGCCAGAAGACGCGGATATGGCTCGGCTCCCACGCCACCGCCGAGGCCGCTGCGCGCGCGCACGACGCCGCGCTGCTCTGCCTCCGGGGCTCGGCGGCCGACCTCAACCTCCCGCTCCGCCTCCCGTTCGACCTGCCCCCCGCGGCCACCATGTCGCCCAAAGCCATCCAGCGCGTGGCCGCCGCCGCCGCCAGCGCCTCCTCCTCCTCCTCCTGCGGCGGTGGCGGCGGCTTGCGCGCGCCGCCGCCCTTCGCGCCACGCGCCGAGAACAACGGCACCAGTGCCTGCAGCGACGGCGACGGCGACGCCACCCCGGCGTCGAGCACCACCAGCTCGCCCACCAGCCACGCCGACGACGTCTCCTCCCCGGACTCCACCGTCAGCAGCGCCGGCGACGTCGACTACTACACCTCGCTCGCGGACATCGACGCCTTCTTCCAGTCGCCCAAGTGCGTGGTGGACTACGCCCTCATGGACCCCTGCAGCGCCTTCTTCGCGCCGGACTCGACGGCGGCCGACGACGACGGCGGCTGCTGCTGGGCCTGGGAGGAGGACGGCGGCGGCATTGCGCTCTGGAGCTTCTCCGCTACACTGGACTGC
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