| TFome pUT5677 | |
|---|---|
| Template | DR811519 |
| Genbank Insert ID | KJ727736 |
| Protein Name | ZmWRKY59 |
| Species | Zea mays |
| TF Family | WRKY |
| Gene id | GRMZM2G031963 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.1368del27 Support in RNA-seq. Present in FlcDNA. Not in functional domain. |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT5677
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B350_F |
| Temperature (ºC) | 66.9 |
| Sequence
|
caccATGGAAGGCCATGTAGCAATG |
3' Primer
| Name | B350_R |
| Temperature (ºC) | |
| Sequence
|
CGTCGGCCTTGGGCGCCA |
PCR Condition
5 l GC buffer (NEB) 1 l primer forward 20mM 1 l pr
Amino Acid Sequence
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MEGHVAMGWKDPNPGQESSMVFQSRAVRPDTVEGHSNEDVEAGFEKHGLSAALNSPQHEGRSLPLTPQFGQNSSPGSSLAERMQARAGFKVPKLNMPFSTVAGDDSSVPGAPSPYLTIPPGLSPATLLESPVFISNALGQASPTTGKLFLFGSTNDNDPIRPGGPPVGDGTDAFSFKPLDLKSSHYTAEVMKEQNTQSSVKTEAKTQAVQEANLLGQLNQQNHDGQTNMNSDGARDSKLSRLASGTGAGNEHASPPDYGQRAEEADAREDYPAAMAVTAPAEDGYSWRKYGQKQVKHSEYPRSYFKCTHPNCLVKKKVERSHEGHVTEIIYKGTHNHPKPTQSRRPGAGAHPLGGGAQADAADNLQGSQANAAEANQAWRAGVQDGVDATSPPSVPGELCDSAASMQVDCAARFGSPEGADVTSAVSDEVDGDDRVTLTHGGANAAEGDELESKRRKLESYAIDMSTASRAVREPRVVIQTTSEVDILDDGYRWRKYGQKVVKGNPNPRSYYKCTQPGCTVRKHVERASHDLKAVITTYEGKHNHEVPAARNSGHPSAAAAPGAGGPRRQEHPSVHDGLMRQHLGGCGVPFGLPPPPPPPRDPLAPMRNYPTYGFTALGAGSTSLPSLPMLPVLAPSSLHQHPLLRHRQAVQAAGLAWRPRPT
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAAGGCCATGTAGCAATGGGGTGGAAGGATCCTAATCCAGGCCAAGAATCTTCCATGGTCTTCCAATCCAGAGCTGTTCGGCCAGACACCGTAGAGGGGCACAGCAACGAGGACGTTGAAGCCGGATTTGAGAAGCATGGCTTGTCAGCCGCCTTAAACTCGCCTCAGCACGAAGGCCGATCACTGCCACTGACCCCACAGTTTGGCCAGAACTCCAGTCCTGGAAGCAGCCTCGCCGAGAGGATGCAGGCAAGAGCCGGATTCAAGGTGCCGAAGCTGAACATGCCTTTCAGCACGGTAGCTGGAGATGATAGTTCAGTGCCAGGAGCTCCATCGCCCTACCTCACGATCCCGCCTGGCTTGAGCCCAGCAACGCTGCTGGAGTCACCTGTCTTCATTTCGAATGCCCTGGGACAAGCCTCACCAACCACGGGGAAGCTATTCTTGTTTGGTAGCACAAATGACAACGATCCTATTCGACCTGGAGGCCCTCCAGTTGGAGATGGCACTGATGCTTTTTCTTTCAAGCCCTTAGATCTGAAATCCTCACACTACACTGCTGAAGTAATGAAGGAACAAAATACGCAGTCTTCTGTGAAGACTGAAGCTAAGACTCAGGCAGTCCAAGAGGCTAATTTGCTGGGCCAACTGAACCAGCAGAATCACGATGGGCAGACAAACATGAATAGTGACGGTGCTCGTGACTCCAAGCTCAGCCGTCTTGCGTCTGGCACCGGTGCAGGCAATGAACACGCATCACCCCCGGATTACGGCCAGAGAGCCGAGGAAGCTGATGCGAGGGAAGACTACCCGGCGGCAATGGCCGTTACAGCGCCAGCGGAGGACGGGTACAGCTGGAGGAAGTACGGGCAGAAGCAGGTGAAGCACAGCGAGTACCCGCGCAGCTACTTCAAGTGCACGCATCCCAACTGCCTGGTGAAGAAGAAGGTTGAGCGCTCGCACGAGGGGCACGTCACGGAGATCATCTACAAGGGAACGCACAACCACCCGAAGCCCACGCAGAGCCGCCGGCCAGGCGCAGGCGCGCACCCGTTGGGCGGCGGTGCGCAGGCGGACGCAGCCGACAACCTGCAGGGGTCACAGGCAAACGCGGCGGAAGCCAACCAGGCGTGGCGTGCGGGTGTCCAGGATGGCGTGGACGCGACGTCGCCCCCGTCGGTTCCCGGTGAGCTCTGTGACTCGGCGGCGTCGATGCAGGTCGACTGCGCGGCCCGGTTTGGGTCCCCCGAGGGCGCGGACGTGACGTCTGCCGTGTCTGACGAGGTGGACGGGGACGACAGGGTGACTCTGACTCATGGCGGTGCCAACGCGGCGGAGGGCGACGAACTGGAATCTAAACGAAGGAAGCTGGAGTCATACGCCATTGACATGAGCACTGCGTCGAGAGCTGTTCGCGAGCCTCGAGTGGTGATCCAAACGACGAGCGAGGTGGACATCCTCGATGACGGCTACCGCTGGCGCAAGTACGGGCAGAAGGTCGTCAAGGGCAACCCGAATCCAAGGAGCTACTACAAGTGCACGCAACCGGGCTGCACGGTGCGGAAGCACGTGGAGCGCGCGTCGCACGACCTCAAGGCCGTGATCACCACGTACGAGGGCAAGCACAACCACGAGGTTCCCGCCGCGAGGAACAGCGGCCACCCGAGCGCCGCGGCGGCGCCAGGCGCGGGTGGCCCGCGCAGGCAGGAGCACCCGTCCGTGCACGACGGCCTGATGAGACAGCACCTGGGCGGCTGCGGCGTGCCGTTCGGCCTGCCGCCGCCGCCGCCGCCGCCAAGGGACCCGCTGGCGCCGATGCGCAACTACCCGACGTACGGCTTCACTGCCCTTGGCGCCGGCTCCACGTCACTGCCGAGCCTGCCGATGCTCCCGGTGTTGGCGCCGTCCTCCCTGCACCAGCACCCGCTGCTGAGGCACCGGCAGGCGGTGCAGGCTGCCGGTCTCGCGTGGCGCCCAAGGCCGACG
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