| TFome pUT5679 | |
|---|---|
| Template | BT066844.1 |
| Genbank Insert ID | KJ727738 |
| Protein Name | ZmMYBR67 |
| Species | Zea mays |
| TF Family | MYB-related |
| Gene id | GRMZM2G049194 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5679
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B323_F |
| Temperature (ºC) | 61.2 |
| Sequence
|
caccATGAAGTTTGACTTATCTATGG |
3' Primer
| Name | B323_R |
| Temperature (ºC) | |
| Sequence
|
CAAAAAACCCGAGCCTT |
PCR Condition
PCR Reaction Mix: Phusion: 5 l GC buffer 1l MgCl2
Amino Acid Sequence
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MKFDLSMEMDDSHGTMVNKSEENDFEKKGTSDPVVYQLVRVEGDGTLVPATEDDVLQFEHFLHNEKVDLPPIEDADHVEEFFNNDCMLLKKPDLEDGSSKLETVEAHTQKLGANLEENILQSLDDSLSPPSDFRIHSQHSDKLLTEQGANVTRQDNASTETTKSTVLNDSCCAEKYKADACSRSVCDTSTDPSVSGVNSSMPDFSILRGEVCLDDLTIRELQEAFRATFGRQTNVKDKIWLKRRITMGLTNSYDVPSSGCVVKDYKIVGKDAKNDMSDMDERAKTGLRATSVVIYPGNEGDSPSSSYYQSEDQEDSSKRVPIHNDEPQGNLLGEQCTDKRTRKPTKRYIEELSDIETHDSTVKNSSPAKRPAHDELLMNPRVAPFNETGSLGTIYPTRKDTFGGFSVHVPYVSRMRRGRPRKDFISFAVTIYDKGPSVEPKGIQPSVEMILVKDGDKGNHLRKVPEVPLKVSSENEHAEAVDSKGVRNLQAKVCSAVSKPKIKHGLTRKHHRAWTLSEVVKLVDGVARYGAGKWSEIRKLSFASYSYRTSVDLKDKWRNLIRATQTQLPAQKDGACPRKTNPSIIPIPPSILLRVKELHELQSQGGGFTAPVKFSGQDGKVLQGKGSGFL
TFome clones do not contain stop codon at 3'end.
CDS
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ATGAAGTTTGACTTATCTATGGAAATGGATGACTCACATGGCACCATGGTTAATAAAAGTGAAGAGAATGACTTTGAAAAGAAAGGCACTTCTGATCCTGTGGTTTATCAGCTAGTTCGGGTTGAAGGTGATGGAACACTTGTTCCTGCTACAGAGGATGATGTTTTGCAGTTTGAACATTTCCTTCACAACGAGAAGGTTGATCTTCCTCCTATTGAAGATGCAGACCATGTGGAAGAGTTTTTCAACAATGACTGCATGTTACTGAAGAAGCCTGACTTGGAAGATGGATCTTCCAAATTGGAGACTGTGGAAGCACACACACAAAAATTGGGTGCCAACTTAGAGGAAAACATATTACAGTCATTGGATGATTCCCTTAGTCCTCCCTCAGACTTTAGAATTCATAGTCAGCATTCAGATAAATTGCTTACAGAACAAGGGGCAAACGTCACTCGACAGGACAATGCTTCAACTGAGACTACAAAATCGACAGTGTTAAATGACTCTTGCTGTGCTGAAAAATATAAGGCTGATGCCTGCTCAAGATCTGTATGCGACACATCTACAGATCCATCTGTTTCGGGAGTTAATAGTTCCATGCCTGATTTTTCCATATTAAGAGGAGAAGTCTGTTTGGATGATCTTACTATTAGAGAACTTCAGGAGGCGTTTAGAGCCACGTTTGGCCGGCAGACTAATGTCAAAGACAAGATATGGCTCAAAAGACGGATTACAATGGGCTTGACTAATTCCTACGATGTTCCAAGTTCAGGTTGTGTAGTTAAAGACTACAAAATTGTTGGCAAGGATGCCAAAAATGATATGTCAGACATGGATGAAAGAGCTAAGACTGGGCTTAGAGCTACTTCTGTGGTTATATATCCAGGAAACGAAGGGGACTCTCCATCTAGCTCTTACTATCAGAGCGAGGACCAGGAAGATTCTTCCAAGAGGGTGCCGATCCATAATGATGAACCACAAGGAAATCTGCTAGGCGAGCAATGCACTGACAAGCGAACTAGAAAGCCAACAAAAAGATACATAGAGGAGCTATCAGACATTGAGACTCATGACTCAACTGTAAAAAATTCTTCACCAGCAAAAAGGCCTGCACATGATGAACTGTTAATGAACCCGCGAGTAGCACCTTTCAATGAGACTGGTTCTCTGGGCACAATCTATCCTACTAGAAAGGATACCTTTGGAGGATTTAGTGTACACGTTCCTTACGTTTCAAGGATGAGAAGGGGGCGCCCTAGGAAGGACTTCATTTCATTTGCGGTAACAATCTACGATAAGGGACCCTCGGTTGAACCTAAAGGGATTCAGCCATCAGTTGAGATGATATTAGTGAAGGATGGTGACAAGGGAAACCACCTGAGGAAAGTACCTGAGGTTCCACTAAAGGTTAGTTCTGAGAACGAGCATGCAGAAGCAGTTGATAGCAAAGGGGTTCGGAACCTACAAGCAAAGGTCTGCAGTGCTGTTTCCAAGCCTAAAATAAAGCATGGTTTAACGCGGAAGCATCATCGAGCATGGACATTGAGTGAGGTCGTGAAGCTGGTGGATGGTGTGGCTCGGTATGGAGCTGGCAAGTGGTCTGAGATTAGAAAACTGTCCTTCGCCTCGTATTCCTACCGCACTTCAGTGGATCTCAAGGATAAATGGCGTAATCTGATCAGAGCAACCCAGACACAGCTTCCGGCGCAAAAAGATGGTGCCTGTCCACGGAAGACTAACCCTTCTATCATACCAATACCACCATCCATTTTGTTGCGAGTGAAGGAGCTACATGAGCTGCAGTCGCAAGGGGGTGGCTTCACAGCCCCAGTCAAGTTCTCTGGGCAGGACGGCAAGGTTCTACAGGGAAAAGGCTCGGGTTTTTTG
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