| TFome pUT5682 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ727741 |
| Protein Name | ZmARF27 |
| Species | Zea mays |
| TF Family | ARF |
| Gene id | GRMZM2G160005 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT5682
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R6-F |
| Temperature (ºC) | 66.2 |
| Sequence
|
caccATGAAGGATCACGGATCGGGCGG |
3' Primer
| Name | R6-R |
| Temperature (ºC) | |
| Sequence
|
GGCCCTCCATGCATTTGCGTC |
PCR Condition
Amino Acid Sequence
Copy to clipboard
MKDHGSGGVTPSPAEGEKKPINSELWHACAGPLVSLPPVGSLVVYFPQGHSEQVAASMHKELDTVPSYPSLPSKLICKLLSLTLHADSETDEVYAQMMLQPVNKYDRDAMLASELGLKQNKQPTEFFCKTLTASDTSTHGGFSVPRRAAEKIFPPLDFTMQPPAQELIAKDLHDISWKFRHIYRGQPKRHLLTTGWSVFVSTKRLLAGDSVLFIRDEKSQLLLGIRRASRPQPALSSSVLSSDSMHIGILAAAAHAAANSSPFTIFYNPRASPSEFVIPLAKYNKALYTQVSLGMRFRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDESTASERRTRVSIWEIEPVATPFYICPPPFFRPKLPKQAGMPDDENEVESAFKRAMPWLADDFALKDVQNALFPGLSLVQWMAMQQNPQMLATASPAVQSQYLTSNALGMQDGIGSVSEDPTKRLTMQAQNIGLPNLQAGSKVDHPAITPLAQHQQQPHHVLQQQQVQPLQQSSAILQQQQAQLLQQNAIHLQQQQEQLQRQQSQPQQQFKATACLQSSDQHKLKEQQPSGGQAASQVHLLNQILQPSSSQLQQLGLPKSPTQRPGLPSLTTMGSLQQSQLPQTPQPQQTAEYHQALLQSQQPQLQQLSQSELQLQLLQKIQQQNLLSQLNPQHQSQLIQQLSQKSQEILQQQVLQHQFGGADAMGQLKHLQQTPLNHNTGSLPPQQLVRSHSALTESEEPSSSTVPSGSRISPINSFSRANQGTRNLPEMPATPHIEHLLQEIQSKTDNRIKNDIQGSKETVHAPNRHLASDQLDASSATSFCLDESPREGFSFPPVCLDNNVQVDPRENFLIAENVDTLMPDALLSRGMSSGKGICNLPSGQRDHRDVENELSSAAFSSQSFGVPDMSFKPGCSSDVAVADGGMASQGLWNSQTQRMRTFTKVQKRGSVGRSIDITRYRGYEDLRHDLACMFGIQGQLEDPYRTDWKLVYVDHENDILLVGDDPWEEFVSCVKSIKILSSVEVQQMSLDGDLGCIPQTQACSASDDANAWRA
TFome clones do not contain stop codon at 3'end.
CDS
Copy to clipboard
ATGAAGGATCACGGATCGGGCGGCGTCACTCCCAGCCCCGCAGAAGGGGAAAAGAAACCCATCAACTCGGAGCTGTGGCACGCTTGTGCGGGGCCCCTGGTGTCGTTGCCCCCGGTGGGCAGTCTCGTCGTATACTTCCCGCAGGGCCATAGTGAACAGGTTGCTGCTTCAATGCATAAGGAGCTGGACACCGTCCCCAGCTACCCATCTCTGCCTTCTAAGTTGATCTGCAAGCTCCTAAGTCTCACCTTACATGCAGATTCTGAAACTGATGAAGTTTATGCCCAGATGATGCTTCAGCCTGTGAACAAATATGATCGAGACGCAATGCTGGCATCTGAACTGGGTCTGAAACAAAACAAGCAACCAACAGAATTCTTTTGCAAGACGTTGACAGCAAGCGACACAAGTACGCATGGGGGATTCTCAGTGCCACGCCGTGCAGCTGAGAAGATATTTCCACCGCTAGACTTTACGATGCAACCACCAGCCCAAGAACTCATTGCCAAGGATCTGCATGATATTTCATGGAAATTTCGACATATCTATCGAGGTCAACCGAAGAGGCATCTTTTGACAACTGGTTGGAGTGTCTTCGTCAGTACAAAAAGGCTGTTAGCCGGTGATTCAGTTCTCTTTATAAGGGACGAGAAATCTCAACTTCTCTTAGGTATAAGGCGTGCTAGCAGACCCCAACCAGCTCTGTCATCATCAGTTTTATCAAGTGACAGCATGCACATTGGAATCCTTGCAGCAGCAGCCCATGCTGCCGCAAATAGTAGCCCATTTACTATTTTCTATAACCCAAGGGCAAGCCCATCAGAATTTGTCATCCCTTTAGCAAAATACAATAAGGCTTTGTATACACAAGTGTCCCTTGGAATGCGATTCAGAATGCTCTTTGAGACAGAGGATTCAGGGGTAAGAAGATACATGGGCACGATCACAGGCATTGGCGACTTAGATCCACTGCGATGGAAGAATTCTCATTGGCGAAACCTTCAGGTTGGTTGGGATGAATCGACTGCATCTGAGAGGCGCACCCGTGTTTCGATATGGGAGATTGAACCAGTTGCTACACCATTTTATATATGCCCACCACCATTTTTCAGGCCAAAGCTTCCTAAGCAAGCAGGAATGCCAGATGATGAAAACGAGGTTGAGAGTGCTTTCAAAAGAGCGATGCCATGGCTTGCTGATGACTTCGCTCTGAAAGATGTCCAAAATGCATTATTTCCAGGCCTGAGCCTAGTTCAATGGATGGCTATGCAGCAAAATCCTCAGATGCTAGCAACTGCTTCCCCTGCTGTACAGTCGCAGTACTTGACCTCTAATGCACTGGGTATGCAGGATGGGATAGGCAGTGTCAGTGAAGATCCAACAAAAAGATTGACTATGCAGGCCCAAAATATTGGCTTACCTAATTTACAGGCTGGTTCGAAAGTGGACCACCCTGCAATTACCCCATTAGCTCAACACCAGCAACAGCCTCACCATGTATTGCAACAACAGCAGGTCCAACCACTGCAGCAAAGTTCTGCGATCCTACAGCAACAGCAAGCCCAGTTGTTGCAGCAAAATGCCATTCACTTGCAGCAGCAGCAAGAACAACTCCAACGGCAACAATCACAGCCGCAGCAACAGTTCAAAGCTACTGCATGTCTTCAGTCATCGGATCAGCACAAGCTGAAAGAGCAACAGCCTTCAGGTGGACAAGCTGCCTCACAAGTACATTTGTTAAACCAGATTTTGCAACCATCTTCATCTCAGCTACAACAGCTGGGTTTACCCAAGTCACCTACTCAGCGCCCAGGGTTACCAAGTCTAACAACAATGGGTTCTTTGCAGCAGTCACAATTACCTCAGACTCCACAGCCGCAACAAACAGCAGAATACCACCAGGCACTCCTACAAAGTCAACAACCCCAACTACAACAGCTATCACAATCAGAACTGCAGCTGCAGCTGCTTCAAAAGATTCAACAACAAAATCTGCTATCTCAACTGAACCCACAGCATCAGTCCCAGCTGATCCAACAATTGTCTCAGAAAAGCCAGGAAATTCTCCAGCAACAAGTTCTTCAACATCAGTTTGGTGGTGCTGATGCCATGGGTCAGCTCAAACATTTACAGCAAACACCTTTGAACCATAATACAGGATCCTTGCCACCCCAGCAACTTGTAAGATCACACTCAGCACTCACTGAGAGCGAAGAACCTTCCAGCTCAACAGTTCCATCTGGCAGCCGTATCTCCCCAATAAATTCATTTAGTAGAGCAAACCAAGGAACCAGAAATTTGCCTGAGATGCCAGCAACACCGCACATTGAGCACTTACTTCAGGAAATTCAAAGTAAGACAGATAATCGAATCAAGAATGATATACAAGGTAGTAAGGAAACGGTCCATGCACCTAATCGTCATCTAGCTTCAGATCAACTTGATGCGTCATCTGCTACCTCCTTTTGCTTAGATGAGAGCCCACGAGAAGGCTTTTCCTTTCCTCCAGTTTGTTTGGATAACAATGTTCAAGTTGATCCAAGAGAGAATTTTCTTATTGCGGAAAATGTGGACACATTAATGCCTGACGCCCTGTTGTCAAGAGGGATGTCGTCTGGAAAGGGTATATGCAATCTACCTTCTGGACAAAGGGATCATAGGGATGTGGAAAACGAGCTATCCTCTGCTGCATTCAGCTCCCAGTCATTTGGTGTGCCGGACATGTCTTTTAAGCCTGGATGTTCAAGTGATGTTGCTGTTGCTGATGGTGGAATGGCAAGCCAAGGACTGTGGAATAGTCAAACACAGCGGATGAGAACCTTCACCAAGGTTCAAAAGCGTGGATCCGTTGGGAGATCAATTGATATAACACGGTACCGAGGTTACGAAGATCTTCGGCATGATCTTGCATGCATGTTCGGTATTCAAGGGCAGCTTGAAGATCCCTACAGGACGGACTGGAAGCTGGTCTATGTTGACCACGAAAATGACATCCTCCTTGTTGGCGACGACCCATGGGAGGAGTTTGTAAGCTGTGTCAAGAGCATTAAAATATTGTCATCTGTTGAAGTACAGCAAATGAGCTTGGATGGTGACCTTGGCTGCATCCCTCAAACTCAAGCCTGTAGTGCTTCTGATGACGCAAATGCATGGAGGGCC
Copyright © 2023 Grassius.org | Last updated: 2023-06-26