| TFome pUT5683 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ727742 |
| Protein Name | ZmARID6 |
| Species | Zea mays |
| TF Family | ARID |
| Gene id | GRMZM2G024976 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT5683
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R13-F |
| Temperature (ºC) | 65.2 |
| Sequence
|
caccATGAGCGAGCTGGTGCGG |
3' Primer
| Name | R13-R |
| Temperature (ºC) | |
| Sequence
|
TGAATCAGGTGGTGCATTATCTTTCTC |
PCR Condition
Amino Acid Sequence
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MSELVRAAAPAAAEEEKKAVAAVTVVAKGKEKAEVTPTVEASSVGDRGRFVAYPSRVAEHTDVVADAARFRAALEGLHTHMGTRLKVPIIGGKDLDLHQLYKEVTSRGGIDKVKAENRWREVTASFIFPATATNASFMLKKYYMSLLYHFEQLYFFRVQGWHQQEIDSRMNSSIEIKTEGQAYHKRKRGINTSSDPASSSDNVDVDVIVDGKFEHGYIVTVIMGSKSTKAILYNCTEEPALPTPEPPVANNSTGLKGVRRRRRRRKKLSTTDPRHPKPNRSGYNFFFQDQHRILKPQYPAQDRLISKMIGERWNNLSPEDKAVYQQRGVEDKERYRTQLAAYKELRTGQPISNAVPIQQRLPLTEVTIDEVDSKVSQDDMLLSNQGYSSSDESDHSGEKNVEDELNTETSPEVSMETTGSPGHPDPSRDGDHFELRRRENPKADEKDNAPPDS
TFome clones do not contain stop codon at 3'end.
CDS
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ATGAGCGAGCTGGTGCGGGCGGCGGCTCCGGCAGCTGCGGAGGAGGAGAAGAAGGCCGTCGCGGCTGTGACTGTGGTGGCGAAAGGAAAAGAGAAGGCGGAGGTGACGCCGACCGTGGAGGCCTCATCGGTCGGCGACAGGGGGAGGTTCGTGGCCTACCCGTCGCGGGTGGCGGAGCACACGGACGTGGTGGCGGACGCCGCGCGCTTCAGGGCGGCGCTCGAGGGGCTGCACACGCACATGGGCACCAGGCTCAAGGTGCCGATCATTGGAGGGAAAGATTTGGATCTTCATCAGCTATATAAGGAGGTCACATCACGGGGCGGTATTGACAAGGTGAAGGCAGAGAATAGATGGAGAGAAGTGACTGCATCATTTATTTTCCCTGCCACTGCGACAAATGCTTCTTTTATGCTGAAGAAATACTATATGTCACTCTTATATCATTTTGAGCAGCTATACTTCTTTAGAGTGCAAGGCTGGCATCAACAAGAAATAGATTCCAGAATGAATTCTTCCATTGAAATTAAAACCGAAGGCCAAGCTTACCACAAAAGAAAAAGGGGCATCAACACATCTTCAGATCCAGCTTCGTCTTCAGATAATGTTGACGTAGATGTGATAGTTGATGGCAAATTTGAACATGGCTACATAGTGACTGTTATTATGGGATCGAAATCCACTAAAGCAATCCTCTATAATTGCACTGAAGAACCTGCTTTGCCAACTCCGGAGCCACCTGTTGCAAATAACAGCACTGGTTTGAAGGGTGTACGTCGCCGAAGACGACGTAGGAAGAAGCTAAGTACAACGGATCCCAGGCACCCCAAACCAAACAGGAGTGGCTATAATTTCTTCTTCCAGGATCAACATAGAATTCTTAAGCCACAATATCCTGCACAAGACAGACTGATCAGTAAAATGATTGGTGAACGATGGAACAATCTAAGTCCTGAAGATAAAGCTGTATATCAGCAAAGAGGTGTAGAGGATAAGGAGCGATACCGGACCCAGTTGGCTGCTTATAAAGAACTGAGGACAGGCCAACCTATCAGCAATGCTGTGCCTATCCAACAGAGGCTCCCTCTGACAGAAGTGACGATTGATGAAGTAGACTCCAAGGTGAGCCAAGATGATATGCTGCTGTCAAACCAAGGGTACAGCAGTAGTGATGAAAGTGACCATTCAGGAGAGAAAAATGTAGAAGACGAGCTCAACACTGAAACATCCCCTGAGGTGAGCATGGAAACAACTGGTTCTCCTGGACACCCTGATCCCTCTCGAGATGGGGACCACTTTGAGTTGCGGCGGAGAGAAAACCCCAAGGCAGATGAGAAAGATAATGCACCACCTGATTCA
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