| TFome pUT5687 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ727746 |
| Protein Name | ZmbHLH157 |
| Species | Zea mays |
| TF Family | bHLH |
| Gene id | GRMZM2G009478 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | exon 3 (291 bp = 97 aa) skipped and intron 7 retained. Protein is truncated within exon 7. Support in RNA-seq |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT5687
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R19-F |
| Temperature (ºC) | 63.8 |
| Sequence
|
caccATGGGCATCCAAGGGAACAAGG |
3' Primer
| Name | R19-R |
| Temperature (ºC) | |
| Sequence
|
GCTGTTGTCCCGTTTATGCCGTTTC |
PCR Condition
Amino Acid Sequence
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MGIQGNKAATHEHDFLSLYAAAAAAAAAKDAPLLLHDSKTPPPSQGHQQQWALPFAGSLQVSPPPQQAQPDRKGRGGGGVMDSGSRSSGGAGFDDEDGLTTRREVSSSLQDLAVRVDWKGGSCSDGGTNQGPNTPRSKHSATEQRRRSKINDRFQILRELLPHNDQKRDKATFLLEVIEYIRFLQEKAQKYEATFPEWNQENPTMLPWVIASLSKAAMLATAVRKGTVNS*LSVSECSFTFLQSNMYFRSFWKNAQASVCC*KMHLPCRVDNAVSIVLNHHLRHACYSCAAE*RPNPRRFPA*PFTFREKWILPWI*FHWETR**SHICSCIRGTGPGRN*SRG*RLLQIIRNSGEYYK*CYISVPTSMDRSVPCG*LCSEK*NA*QPGVGNR*RNDQCVQYLFPRVTYLVVSRPSELRCGSVPNQHLCADQPGKACWQEICICWWCILQLQGTTSRSSI**RTRRPSRDNVGS*R*RSPPPRDETA*TGQQ
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGCATCCAAGGGAACAAGGCGGCGACGCACGAGCACGACTTCCTCTCGTTGTACGCCGCCGCCGCCGCGGCCGCCGCCGCCAAGGACGCTCCTCTCCTGCTCCACGACTCCAAGACGCCGCCTCCTTCTCAAGGGCACCAGCAGCAGTGGGCGCTCCCCTTCGCCGGCTCCTTGCAGGTCAGTCCGCCGCCGCAGCAGGCGCAACCGGATAGGAAGGGCCGCGGCGGAGGCGGGGTCATGGATTCAGGCTCCAGATCCAGCGGCGGAGCCGGGTTCGACGACGAAGACGGCCTCACCACGCGCCGCGAGGTCTCCTCCTCGCTCCAAGATTTGGCAGTAAGGGTGGATTGGAAGGGTGGGAGCTGCAGCGATGGCGGTACAAATCAGGGGCCAAACACACCACGGTCAAAACACTCGGCCACAGAGCAGCGCAGACGCAGCAAGATCAATGATAGGTTTCAGATACTTAGGGAGCTGTTGCCACACAATGATCAAAAGAGAGACAAAGCAACATTTCTCTTGGAGGTTATTGAATATATACGTTTTTTGCAAGAGAAAGCGCAAAAATACGAGGCAACATTCCCAGAATGGAACCAAGAAAATCCAACGATGCTTCCGTGGGTAATTGCTTCTTTATCGAAAGCTGCAATGCTTGCTACTGCCGTTAGGAAAGGAACAGTCAATTCATGATTGTCTGTGTCTGAATGTAGTTTTACATTCTTGCAGTCAAATATGTATTTTCGATCATTCTGGAAAAATGCACAGGCAAGTGTTTGTTGCTAAAAAATGCATTTGCCTTGCCGGGTCGATAATGCTGTGTCAATAGTTTTGAACCATCATCTTAGGCATGCTTGTTATTCTTGTGCTGCAGAGTAAAGGCCAAATCCCAGGAGATTCCCCGCCTGACCCTTCACATTTCGTGAGAAATGGATCCTCCCCTGGATCTAATTTCACTGGGAAACTCGATGATAATCACACATCTGCAGCTGCATCAGGGGCACTGGACCAGGCAGAAACTAATCACGTGGCTAGCGGCTGCTACAGATCATCAGAAACTCCGGCGAATATTACAAATAATGCTATATCTCAGTCCCAACCTCAATGGACAGGTCCGTCCCCTGTGGATGACTCTGCAGTGAAAAGTGAAATGCTTAACAGCCAGGAGTTGGCAATAGATGAAGGAATGATCAGTGTGTCCAGTACCTATTCCCAAGAGTTACTTATCTCGTTGTCTCACGCCCTTCAGAGCTCAGGTGTGGATCTGTCCCAAACCAGCATCTCTGTGCAGATCAACCTGGGAAAGCGTGCTGGCAAGAGATCTGCATCTGCTGGTGGTGTATCCTCCAACTCCAAGGAACCACCAGCCGATCCAGCATCTAGTAACGAACTAGGCGGCCATCACGTGACAACGTTGGGAGCTAGCGCTGACGGTCTCCTCCCCCACGCGACGAAACGGCATAAACGGGACAACAGC
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