| TFome pUT5706 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ727761 |
| Protein Name | ZmNAC35 |
| Species | Zea mays |
| TF Family | NAC |
| Gene id | GRMZM2G179049 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT5706
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R105F |
| Temperature (ºC) | 69.0 |
| Sequence
|
caccATGGAGAGACCTGCGCAGGCGC |
3' Primer
| Name | R105R |
| Temperature (ºC) | |
| Sequence
|
TTGTATGGTACCAGAAAAAGGTTGGGCTACG |
PCR Condition
Amino Acid Sequence
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MERPAQAPTQLPPGFRFHPTDEELVVLYLRRKALARPLPAAVIPVVHDVAGLDPWDLPGASEGEGYFFSLRRAPATGRGSRRRRAGSGYWKATGKEEPVFLQCGGGGVGGKRQLLVGGKTALAFHRSEPSPPSSSRTGWVMHEYRLAAASRGVAEQRKKNASHGCCDAEPGEWVVCRVFLKKGARRSRPGRDGNHSRALRHRASAAPPQLREGVGGRQPLLFPAPQSPSSSCVTGVTDLSDEEDEVSSGSITRVCTSERGLMGILSVPSPLSHGPVAQPFSGTIQ
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAGAGACCTGCGCAGGCGCCCACGCAGCTGCCGCCGGGGTTCCGGTTCCACCCCACCGACGAGGAGCTGGTAGTGCTGTACCTCCGCCGGAAGGCCCTGGCTCGCCCGCTGCCGGCCGCCGTCATCCCCGTCGTCCACGACGTCGCCGGGCTCGATCCATGGGACCTCCCTGGGGCAAGCGAAGGGGAGGGTTACTTCTTTAGCCTGCGGCGAGCGCCGGCGACCGGCCGTGGCAGCCGCAGGAGGAGAGCTGGGAGCGGGTACTGGAAGGCCACGGGCAAGGAGGAGCCGGTGTTCCTGCAGTGCGGCGGCGGCGGCGTAGGCGGCAAGAGGCAGCTGCTCGTGGGCGGCAAGACGGCGCTCGCCTTCCACCGCAGTGAGCCGTCGCCGCCGTCGTCGTCGCGGACAGGCTGGGTCATGCACGAGTACCGGCTCGCCGCCGCGTCCCGCGGCGTGGCCGAGCAGAGGAAGAAGAATGCGAGCCACGGTTGTTGCGATGCTGAGCCGGGAGAGTGGGTCGTGTGCCGGGTCTTTCTGAAGAAGGGAGCAAGAAGAAGCAGGCCGGGCCGGGATGGTAACCACAGCAGAGCTCTGAGACACCGCGCATCCGCTGCGCCGCCGCAGCTCCGGGAAGGTGTGGGAGGACGGCAGCCGTTGCTGTTCCCGGCGCCACAGTCGCCGTCCTCAAGCTGTGTCACCGGCGTTACCGACTTGTCAGACGAAGAAGACGAAGTCAGCAGCGGCAGCATAACAAGAGTTTGCACCTCAGAGAGAGGCCTGATGGGGATTTTAAGTGTTCCCTCCCCCCTCTCTCATGGTCCCGTAGCCCAACCTTTTTCTGGTACCATACAA
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